Random Primers DNA Labeling System
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Invitrogen™

Random Primers DNA Labeling System

ランダムプライマーDNAラベリングシステムは、DNA、特にフラグメント––[α-32 P]-dCTP–25 ngを使用して、1回の反応で>109 cpm/µgのコントロールDNAを生成します。性能と品質のテスト:放射性標識ヌクレオチドの組み込みは、ランダムプライマー標識反応でコントロールDNAを使用して検証されます詳細を見る
製品番号(カタログ番号)数量
1818701330反応
製品番号(カタログ番号) 18187013
価格(JPY)
57,600
Each
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数量:
30反応
ランダムプライマーDNAラベリングシステムは、DNA、特にフラグメント<1 kbを放射性標識するのに最適です。ランダムプライマーDNAラベリングシステム:
––[α-32 P]-dCTP
–25 ngを使用して、1回の反応で>109 cpm/µgのコントロールDNAを生成します。


性能と品質のテスト:放射性標識ヌクレオチドの組み込みは、ランダムプライマー標識反応でコントロールDNAを使用して検証されます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法放射活性
最終産物タイププローブ(標識DNA)
標識または色素を含む不可
標識法直接標識
標識標的DNA(一般)
標識または色素32P(リン-32)
製品タイプDNA Labeling System
数量30反応
出荷条件氷水またはドライアイスでの出荷が承認されています
Unit SizeEach
組成および保存条件
ランダムプライマーDNAラベリングシステムのコンポーネントは、右の表に記載されています。-20℃で保存

よくあるご質問(FAQ)

How can I label COT-1 DNA?

Probes can be labeled with 32P by random primer or nick translation procedures using the Random Primers DNA Labeling System (Cat. No.18187-013) or Nick Translation System (Cat. No. 18160-010). Biotinylated COT-1 DNA can be prepared by nick translation with the BioNick Labeling System (Cat. No. 18247-015) or by the BioPrime DNA Labeling System (Cat. No. 18094-011). Improved results can be obtained when the COT-1 DNA is first ligated to itself to provide an optimum template.

引用および参考文献 (12)

引用および参考文献
Abstract
Human biliverdin reductase is a leucine zipper-like DNA-binding protein and functions in transcriptional activation of heme oxygenase-1 by oxidative stress.
Authors:Ahmad Zulfiqar; Salim Mohammad; Maines Mahin D;
Journal:J Biol Chem
PubMed ID:11773068
Human biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. A domain of biliverdin reductase (BVR) has primary structural features that resemble leucine zipper proteins. A heptad repeat of five leucines (L(1)--L(5)), a basic domain, and a conserved alanine ... More
Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways.
Authors: Ueda Takeshi; Bruchovsky Nicholas; Sadar Marianne D;
Journal:J Biol Chem
PubMed ID:11751884
'The androgen receptor (AR) is a ligand-activated transcription factor that mediates the biological responses of androgens. However, non-androgenic pathways have also been shown to activate the AR. The mechanism of cross-talk between the interleukin-6 (IL-6) and AR signal transduction pathways was investigated in LNCaP human prostate cancer cells. IL-6 induced ... More
PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis.
Authors: Ren Delin; Collingwood Trevor N; Rebar Edward J; Wolffe Alan P; Camp Heidi S;
Journal:Genes Dev
PubMed ID:11782439
'To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in gamma2 expression but had no ... More
Interaction of the Poliovirus Receptor CD155 with the Dynein Light Chain Tctex-1 and Its Implication for Poliovirus Pathogenesis.
Authors: Mueller Steffen; Cao Xuemei; Welker Reinhold; Wimmer Eckard;
Journal:J Biol Chem
PubMed ID:11751937
'The cellular receptor for poliovirus CD155 (or PVR) is the founding member of a new class of membrane-associated immunoglobulin-like proteins, which include the mouse tumor-associated antigen E4 (Tage4) and three proteins termed ' ... More
A trypanosome mitochondrial RNA polymerase is required for transcription and replication.
Authors: Grams Jayleen; Morris James C; Drew Mark E; Wang Zefeng; Englund Paul T; Hajduk Stephen L;
Journal:J Biol Chem
PubMed ID:11859084
'Understanding mitochondrial transcription is a requisite first step toward understanding the regulation of mitochondrial gene expression in kinetoplastids. Here we report the identification and functional characterization of a mitochondrial RNA polymerase (mtRNAP) from Trypanosoma brucei, the first trans-acting factor involved in kinetoplast mitochondrial transcription to be identified. Using sequences conserved ... More