Lipofectin™ Transfection Reagent

Citations & References (54)

Invitrogen™

Lipofectin™ Transfection Reagent

Name: Lipofectin™ Transfection Reagent
SKU: 18292037

テクニカルサポートオーダーサポート

表示を変更

製品番号（カタログ番号）	数量
18292037	4 mL
18292011	1 mL

2 オプション

製品番号（カタログ番号） 18292037

価格（JPY）

277,200

Each

お問い合わせください ›

数量:

4 mL

Lipofectin®トランスフェクション試薬は、内皮細胞のトランスフェクションに最適な試薬です。Lipofectin®トランスフェクション試薬は、DNA、RNA、オリゴヌクレオチドの哺乳類細胞へのトランスフェクション、DNAの植物のプロトプラストへのトランスフェクションにも最適です。Lipofectin試薬は、HeLa細胞のトランスフェクションにおいて、PLUS®試薬と組み合わせて良好に機能することも示されています。Lipofectin®トランスフェクション試薬は、膜フィルタでろ過した陽イオン性脂質N-[1-(2,3-dioleyloxy)propyl]-n,n,n-塩化トリメチルアンモニウム（DOTMA）およびジオレオイルホスファチジルエタノールアミン（DOPE）の1：1（w／w）のリポソーム製剤です。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

使用対象（アプリケーション）Transfection

高スループット適合性ハイスループット非対応（手動）

内容Lipofectinトランスフェクション試薬のバイアル（各1 mL）4本

製品ラインLipofectin

製品タイプトランスフェクション試薬

数量4 mL

適合血清不可

出荷条件湿氷

細胞タイプ株化細胞, 初代細胞, トランスフェクションが困難な細胞

フォーマット6ウェルプレート、12ウェルプレート、24ウェルプレート、48ウェルプレート、96ウェルプレート、フラスコ

サンプルタイププラスミドDNA

Transfection Technique脂質ベースのトランスフェクション

Unit SizeEach

組成および保存条件

各ユニットには、試薬のバイアル（1 ml）が1本または複数入っています。4℃で保存。Lipofectin™トランスフェクション試薬は、適切に保存した場合、6カ月間安定です。

よくあるご質問（FAQ）

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectin™ Transfection Reagent, 4 mL FAQs

引用および参考文献 (54)

引用および参考文献

Abstract

Transfection techniques for producing recombinant baculoviruses.

Authors:Trotter KM, Wood HA

Journal:Methods Mol Biol

PubMed ID:7620563

A role for calcium in stabilizing transport vesicle coats.

Authors: Ahluwalia J P; Topp J D; Weirather K; Zimmerman M; Stamnes M;

Journal:J Biol Chem

PubMed ID:11435443

'Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N'',N''-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle ... More

Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.

Authors: Iwabuchi K; Li B; Massa H F; Trask B J; Date T; Fields S;

Journal:J Biol Chem

PubMed ID:9748285

'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated ... More

Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.

Authors: Schmedtje J F Jr; Ji Y S; Liu W L; DuBois R N; Runge M S;

Journal:J Biol Chem

PubMed ID:8995303

'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction ... More

Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.

Authors: Yoshimura K; Rosenfeld M A; Nakamura H; Scherer E M; Pavirani A; Lecocq J P; Crystal R G;

Journal:Nucleic Acids Res

PubMed ID:1377820

'As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed of a firefly ... More

View all Lipofectin™ Transfection Reagent, 4 mL citations