How long does the labeling take when using the RadPrime DNA Labeling system?
Typically labeling is complete in 10 minutes, and purifiation of the probe is not usually required.
引用および参考文献 (6)
引用および参考文献
Abstract
Molecular characterization of the homo-phytochelatin synthase of soybean Glycine max: relation to phytochelatin synthase.
Authors: Oven Matjaz; Page Jonathan E; Zenk Meinhart H; Kutchan Toni M;
Journal:J Biol Chem
PubMed ID:11706029
'The phytochelatin homologs homo-phytochelatins are heavy metal-binding peptides present in many legumes. To study the biosynthesis of these compounds, we have isolated and functionally expressed a cDNA GmhPCS1 encoding homo-phytochelatin synthase from Glycine max, a plant known to accumulate homo-phytochelatins rather than phytochelatins upon the exposure to heavy metals. The ... More
Two Proteins Essential for Apolipoprotein B mRNA Editing Are Expressed from a Single Gene through Alternative Splicing.
Authors: Dance Geoffrey S C; Sowden Mark P; Cartegni Luca; Cooper Ellen; Krainer Adrian R; Smith Harold C;
Journal:J Biol Chem
PubMed ID:11815617
'Apolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A ... More
Activation of the phosphatidylinositol 3-kinase/Akt signaling pathway by retinoic acid is required for neural differentiation of SH-SY5Y human neuroblastoma cells.
'Retinoic acid (RA) induces neural differentiation of SH-SY5Y neuroblastoma cells. We show that the mRNA levels of the differentiation-inhibiting basic helix-loop-helix transcription factors ID1, ID2, and ID3 are down-regulated during RA-induced differentiation of SH-SY5Y cells. The levels of ID proteins decreased in parallel to the observed transcriptional repression. The expression ... More
Novel Alternative Splicings of BPAG1 (Bullous Pemphigoid Antigen 1) Including the Domain Structure Closely Related to MACF (Microtubule Actin Cross-linking Factor).
'BPAG1 (bullous pemphigoid antigen 1) was originally identified as a 230-kDa hemidesmosomal protein and belongs to the plakin family, because it consists of a plakin domain, a coiled-coil rod domain and a COOH-terminal intermediate filament binding domain. To date, alternatively spliced products of BPAG1, BPAG1e, and BPAG1n are known. BPAG1e ... More
A DEAD-Box Protein Functions as an ATP-Dependent RNA Chaperone in Group I Intron Splicing.
Authors: Mohr Sabine; Stryker John M; Lambowitz Alan M;
Journal:Cell
PubMed ID:12086675
The Neurospora crassa CYT-18 protein, the mitochondrial tyrosyl-tRNA synthetase, functions in splicing group I introns by inducing formation of the catalytically active RNA structure. Here, we identified a DEAD-box protein (CYT-19) that functions in concert with CYT-18 to promote group I intron splicing in vivo and vitro. CYT-19 does not ... More