Detoxi-Gel™ Endotoxin Removing Columns, 1 mL
Thermo Scientific™

Detoxi-Gel™ Endotoxin Removing Columns, 1 mL

Thermo Scientific Detoxi-Gel Endotoxin Removing Gelは、アフィニティーレジンビーズに固定化されたポリミキシンBリガンドを使用して、抗体またはタンパク質サンプルからエンドトキシンを結合して抽出します。Detoxi-Gel Endotoxin詳細を見る
製品番号(カタログ番号)数量
203445カラム
製品番号(カタログ番号) 20344
価格(JPY)
46,600
Each
数量:
5カラム
一括またはカスタム形式をリクエストする
Thermo Scientific Detoxi-Gel Endotoxin Removing Gelは、アフィニティーレジンビーズに固定化されたポリミキシンBリガンドを使用して、抗体またはタンパク質サンプルからエンドトキシンを結合して抽出します。

Detoxi-Gel Endotoxin Removing Gelはサンプルからエンドトキシン(リポ多糖類(LPS))を迅速に除去します。低濃度のエンドトキシンでも細胞や生物には有毒で、生体サンプルを添加する前に除去する必要があります。

Detoxi-Gel Endotoxin Removing Gelの特長:

•樹脂は固定化ポリミキシンBを使用し、リピドA領域と結合してパイロジェンを除去します。
• タンパク質溶液、細胞培養培地、薬理成分や水性バッファーを含む溶液からエンドトキシンのレベルを低下させます。
•1 mLのゲルは>10,000 EUの対象サンプルから9,995 EUのLPSを除去します(99%を超える効率)。
•1 mLのゲルは大腸菌株055:BSからリポ多糖4,000~6,000 EUを結合します。
• タンパク質の回収はタンパク質の種類と濃度に依存し、実証実験が必要です。
• 安定性が高く再利用が可能。樹脂は、パイロジェンをあまり含まない水による1%デオキシコール酸溶液でエンドトキシンを除去して再生できます。10回再生後も結合能力を保持します。

関連製品
Detoxi-Gel™ Endotoxin Removing Gel
研究用途にのみご使用ください。診断目的には使用できません。
仕様
カラムタイプアフィニティー、アガロースレジン
概要Detoxi-Gelエンドポイント除去カラム、1 mL
フォーマットSpin Column
数量5カラム
固定相ポリミキシンB
ターゲットエンドトキシン除去
容量(メートル法)1 mL
形状15 mLコニカル、スピンカラム
製品ラインDetoxi-Gel
タイプカラム
Unit SizeEach
組成および保存条件
4℃で保存。

よくあるご質問(FAQ)

Why must I regenerate the Detoxi-Gel Endotoxin Removing Gel column before and after each use?

It is possible that the column may have been contaminated with endotoxin during storage and handling. Equilibrating the column with 1% sodium deoxycholate followed by pyrogen-free washing ensures that the column is free of endotoxin before the sample is added. The column must be regenerated following endotoxin removal from a sample to make sure the endotoxin that has bound to the column from the sample is washed off.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the void volume of the Detoxi-Gel Endotoxin Removing Gel column?

The Thermo Scientific 1 mL Detoxi-Gel Column has a void volume of 0.3-0.5 mL. This is the volume of solution that must be collected from the column before the sample will begin to come off the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the protocol for using Detoxi-Gel Endotoxin Removing Gel?

1. Equilibrate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water or buffer of choice.
2. Apply sample, being sure not to exceed the binding capacity of the gel.
3. Collect sample by gravity flow by applying additional pyrogen-free water or buffer.
4. Regenerate the column with 5 column volumes of 1% sodium deoxycholate in pyrogen-free water, followed by 3 column volumes of pyrogen-free water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity of Detoxi-Gel Endotoxin Removing Gel?

Detoxi-Gel Support has the capacity to bind up to 2µg LPS/mL of gel. Because one endotoxin unit (EU) is approximately 0.1 ng/mL, the gel has the potential for binding up to 20,000 EU/mL of gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is Detoxi-Gel Endotoxin Removing Gel, and how does it work?

Detoxi-Gel Support consists of 6% cross-linked beaded agarose that has Polymixin B immobilized onto it. Polymixin B is an antibiotic that contains a cationic cyclopeptide with a fatty acid chain that can neutralize the biological activity of endotoxins by binding to the lipid A portion of the bacterial lipopolysacharide (LPS). After the endotoxin from a sample binds to the immobilized Polymixin B, the sample can be collected and the endotoxin can be eluted using 1% sodium deoxycholate.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.