Leibovitz's L-15 Medium, no phenol red

Citations & References (2)

Gibco™

Leibovitz's L-15 Medium, no phenol red

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.詳細を見る

製品番号（カタログ番号）	数量
21083027	500 mL

製品番号（カタログ番号） 21083027

価格（JPY）

9,300

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500 mL

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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO₂ equilibration.

This L-15 is modified as follows:

With	Without
• Galactose	• Glucose
• L-glutamine	• Phenol Red
• Sodium Pyruvate	• HEPES
	• Sodium Bicarbonate

The complete formulation is available.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco™ L-15 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.

L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

仕様

細胞株HEP-2 monkey kidney cells

細胞タイプexplants of embryonic and adult human tissue

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプLeibovitz's L-15 Medium

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin

形状Liquid

無菌性Sterile-filtered

添加剤ありGlutamine, Sodium Pyruvate, Galactose

添加剤なしNo Glucose, No HEPES, No Phenol Red

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Leibovitz L-15 medium contain sodium bicarbonate?

No, Leibovitz L15 medium (liquid or powder) does not contain sodium bicarbonate (the formulation for each catalog number is published online). Developed for the support of cell growth in non CO2-equilibrated environments, L-15 formulations were developed without a sodium bicarbonate buffer system. L-15 medium is buffered by phosphates and free-base amino acids (L-arginine primarily, but also L-histidine and L-cysteine). Galactose and sodium pyruvate are used to replace glucose in order to reduce the production of acidic metabolites.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the Leibovitz media you offer?

We offer the following Leibovitz media:

- Leibovitz's L-15 Medium, powder: Cat. Nos. 41300039 (10x 1L) and 41300070 (1x 10L)
- Leibovitz's L-15 Medium, no phenol red: Cat. No 21083027 (1x 500 mL)
- Leibovitz's L-15 Medium: Cat. Nos.11415064 (1x 500 mL) and 11415114 (10x 500 mL) (for sale in N. America, Latin America, and Asia-Pacific regions; Cat. No. 11415049 (for sale in Europe, Africa, and the Middle East
- Leibovitz's L-15 Medium with GlutaMAX Supplement: Cat. No. 31415029 (1x 500 mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Leibovitz's L-15 Medium, no phenol red FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Ancient origin of somatic and visceral neurons.

Authors:Nomaksteinsky M, Kassabov S, Chettouh Z, Stoeklé HC, Bonnaud L, Fortin G, Kandel ER, Brunet JF

Journal:BMC Biol

PubMed ID:23631531

'A key to understanding the evolution of the nervous system on a large phylogenetic scale is the identification of homologous neuronal types. Here, we focus this search on the sensory and motor neurons of bilaterians, exploiting their well-defined molecular signatures in vertebrates. Sensorimotor circuits in vertebrates are of two types: ... More

Antiapoptotic activity of the free caspase recruitment domain of procaspase-9: a novel endogenous rescue pathway in cell death.

Authors: Stephanou Anastasis; Scarabelli Tiziano M; Knight Richard A; Latchman David S;

Journal:J Biol Chem

PubMed ID:11825888

Mitochondrial injury initiates proteolytic processing of procaspase-9 into the large and small subunits, leading to apoptotic cell death. Here we show that the free caspase recruitment domain (CARD) released by procaspase-9 processing activates nuclear factor kappaB expression. A procaspase-9 construct with a point mutation that abrogates the release of the ... More