2-Mercaptoethanol

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent詳細を見る

製品番号（カタログ番号）	数量
21985023	50 mL

製品番号（カタログ番号） 21985023

価格（JPY）

24,000

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50 mL

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration of 55 mM in Dulbecco's phosphate buffered saline (DPBS).

cGMP manufacturing and quality system
Gibco Reducing Agent is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプMammalian Cells

濃度55 mM

数量50 mL

品質保持期間36 Months

出荷条件Room Temperature

形状Liquid

製品タイプBME

pH6 to 8

Unit SizeEach

組成および保存条件

Storage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture

よくあるご質問（FAQ）

I've purchased Gibco 2-Mercaptoethanol (Cat. No. 21985023). Should I add it to my cell culture medium right before every use or can I add it to the bulk medium in advance?

The 2-Mercaptoethanol must be added to the medium immediately before use because activity rapidly declines in diluted form. You can supplement enough medium needed for that day.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.

Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:11248053

'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More

Somatic coding mutations in human induced pluripotent stem cells.

Authors:Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K,

Journal:Nature

PubMed ID:21368825

Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unknown whether the genome remains unchanged at the single nucleotide level. Here we show that 22 human induced pluripotent stem (hiPS) cell lines ... More