EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)
Citations & References (7)
Thermo Scientific™

EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Thermo Scientific Pierce EMCSは、中程度の長さ（9.4オングストローム）の両端にNHSエステル基およびマレイミド反応基を含むアミン基-スルフヒドリル基間架橋剤です。EMCSの特徴：•反応基：NHSエステルとマレイミド• 以下と反応：アミノ基およびスルフヒドリル基• C6-ホモログの脂肪族スペーサーシリーズ詳細を見る
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製品番号（カタログ番号）数量
2230850 mg
製品番号（カタログ番号） 22308
価格（JPY）
34,600
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Ends: 26-Dec-2025
57,800
割引額 23,200 (40%)
Each
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数量:
50 mg
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Thermo Scientific Pierce EMCSは、中程度の長さ（9.4オングストローム）の両端にNHSエステル基およびマレイミド反応基を含むアミン基-スルフヒドリル基間架橋剤です。

EMCSの特徴：

•反応基：NHSエステルとマレイミド
• 以下と反応：アミノ基およびスルフヒドリル基
• C6-ホモログの脂肪族スペーサーシリーズ、AMAS、BMPS、GMBS、およびEMCSを含む
• NHSエステルの末端はpH 7～9で第一級アミンと結合し、安定したアミド結合を形成
• マレイミドはpH 6.5～7.5で-Sh基と反応し、安定したチオエーテル結合を形成
• 非切断性
• 不水溶性（DMFまたはDMSOで最初に溶解）。Sulfo-EMCSと比較
•結合とGMBSの比較で球体が増加
•短脂肪族スペーサーは免疫応答を誘発する可能性が低い

製品リファレンス：
架橋剤アプリケーションガイド - この製品に関する最近の文献参照情報を検索
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞透過性Yes
概要EMCS
形状Powder
標識法化学的標識
分子量308.29
PEG化No
製品ラインPierce
数量50 mg
反応性部分マレイミド、NHSエステル
出荷条件Ambient
溶解性DMF、DMSO
スペーサーアーム長9.4 Å
水溶性No
化学反応性Amine-Sulfhydryl
CleavableNo
クロスリンカータイプヘテロ三官能性
フォーマットStandard
製品タイプ架橋剤
スペーサー短鎖
Unit SizeEach
組成および保存条件
4℃で乾燥した場所に保存。室温で出荷

よくあるご質問（FAQ）

Can you provide the shelf-life for EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)?

EMCS (N-ε-malemidocaproyl-oxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用および参考文献 (7)

引用および参考文献
Abstract
Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12.
Authors:Chen Z, Akin BL, Jones LR
Journal:J Biol Chem
PubMed ID:17548345
'Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-bound state. PLB and Ca(2+) binding to SERCA2a are mutually exclusive, and PLB inhibition of ... More
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.
Authors:Härmä H, Tarkkinen P, Soukka T, Lövgren T
Journal:Clin Chem
PubMed ID:11067810
Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum ... More
Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.
Authors:Akin BL, Jones LR
Journal:J Biol Chem
PubMed ID:22247554
Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker, ... More
PNA microarrays for hybridisation of unlabelled DNA samples.
Authors:Brandt O, Feldner J, Stephan A, Schröder M, Schnölzer M, Arlinghaus HF, Hoheisel JD, Jacob A
Journal:Nucleic Acids Res
PubMed ID:14500847
Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process ... More
Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum.
Authors:Chen Z, Stokes DL, Jones LR
Journal:J Biol Chem
PubMed ID:15644311
The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach. Leu(31) of PLB, located at the cytoplasmic membrane boundary, is a critical amino acid shown previously to be ... More
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