Opti-MEM™ Reduced Serum Medium, powder

Name: Opti-MEM™ Reduced Serum Medium, powder
SKU: 22600134

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation詳細を見る

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製品番号（カタログ番号）	数量
22600134	10 L
22600050	10 x 1 L

2 オプション

製品番号（カタログ番号） 22600134

価格（JPY）

37,100

Each

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数量:

10 L

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Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.

This Opti-MEM™ is modified as follows:

With

• L-glutamine

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.

Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.

仕様

細胞株Sp2, AE-1, CHO, BHK-21, and HEK

細胞タイプPrimary Fibroblasts

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco, Opti-MEM

製品タイプOpti-MEM I

数量10 L

品質保持期間30 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin

形状Powder

無菌性Sterile-filtered

添加剤ありGlutamine, Phenol Red

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 30 months from date of manufacture

よくあるご質問（FAQ）

Do you offer an alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050)?

The direct alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050) is Opti-MEM Reduced Serum Medium powder (Cat. No. 22600134). The product is the same but comes in a 10 L volume. Therefore, you might consider reconstituting the powder and then aliquoting the resulting media into smaller bottles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) should I use to reconstitute the media?

We recommend reconstituting Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) at 13.592 g/L.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ Reduced Serum Medium, powder, 10 L FAQs