Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible
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Thermo Scientific™
Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible
このBCAタンパク質アッセイキットは、当社の一般的なPierce BCAタンパク質アッセイの還元剤適合性バージョンです。このキットを使用すると、ジスルフィド還元剤を含むサンプル中のタンパク質濃度を測定できます。使用可能なすべてのBCAタンパク質アッセイを比較›BCAタンパク質アッセイキットの特徴—還元剤適合性には以下が含まれます。•適合性— 5 mM DTT詳細を見る
対応範囲:25 µLサンプルを使用した試験管アッセイ 250回 • BCA試薬A、250 mL • BCA試薬B、25 mL • 適合性試薬、10×20 mg • 再構成バッファー、15 mL • アルブミン標準アンプル、2 mg/mL、10×1 mL
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よくあるご質問(FAQ)
Which Thermo Scientific protein assay is the best or the most reliable?
The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).
How many assays can be performed with each BCA-RAC kit?
The BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) has sufficient reagents for 250 samples or standards of 25µL each, and the Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) can be used to assay 1,000 samples or standards of 9µL each.
Can I increase the volumes of the sample and compatibility reagent in the microplate assay when using the BCA-RAC?
No. Increasing the sample and compatibility reagent volumes will require increasing the BCA working reagent, resulting in a volume that exceeds the well capacity. There must be space remaining in the well to allow mixing and prevent the solution from contacting the sealing tape.
There are two different formats: the BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) for microcentrifuge tube (1.5ml) assays and Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) for 96-well plates. The tube assay is compatible with 5mM DTT, 10mM TCEP and 35mM 2ME, and the microplate assay is compatible with 5mM DTT, 10mM TCEP and 25mM 2ME.
Authors:Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC
Journal:Anal Biochem
PubMed ID:3843705
Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The ... More