Will the reducing agent in my sample interfere with the Glycoprotein Carbohydrate Estimation assay?
Yes. Reducing agents interfere with this assay. Please see the list of compatibility of other commonly used reagents below:
Compatible: EDTA, Urea (<1.0M), Guanidine HCl (<1.0M), DMSO (<10%), PBS
Not Compatible: Reducing Agents, Tris, Glycerol
Will the detergent in my sample interfere with the Glycoprotein Carbohydrate Estimation assay?
Most commonly used detergents do not interfere; however, detergents with sugar derivatives, such as octyl-b-glusoside (OG) and octyl-b-thioglucopyranoside (OTG), can contribute to the signal, resulting in an erroneously high percent carbohydrate value.
What is the difference between Glycoprotein Carbohydrate Estimation Kit and the Thermo Scientific Glycoprotein Staining Kit (#24562)?
Glycoprotein Carbohydrate Estimation Kit estimates the carbohydrate content of glycoproteins in solution; however, the Thermo Scientific Glycoprotein Stain directly stains oxidized glycoprotein on gels and nitrocellulose membranes and does not estimate carbohydrate content.