Are the magnets on the KingFisher Flex Purification System magnetic head strong enough to disturb nearby laboratory equipment?
We do not recommend placing the KingFisher Flex Purification System in close proximity to magnetic tapes, computer discs or other magnetic storage systems, such as credit cards, as these can be damaged by the strong magnetic field of the KingFisher Flex magnetic heads. Do not hold the KingFisher Flex magnetic heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex magnetic heads. People wearing a pacemaker or metallic prostheses should also not use this product as these items could be affected or damaged by close contact with a strong magnetic field.
Can I use magnetic beads for protein isolation with the KingFisher Flex Purification System? If so, do you recommend any specific magnetic beads and are KingFisher Flex script protocol (.bdz) files available for these applications?
Any kind of protein purification can be done on the KingFisher Flex or Duo Prime as long as it is based on magnetic beads. Some template protocols for different magnetic beads are available for the KingFisher Flex and Duo Prime at this website: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/automated-protocols-software.html?open=protein.
Please know that these templates are only a starting point and each protein and application is unique so optimization will be required. This optimization and protocol modification is the responsibility of the end user.
Where can I find Bindlt script protocol (.bdz) files for use of MagMAX kits with the KingFisher Flex Purification System and KingFisher Duo Prime Purification System?
The best place to download KingFisher Flex and KingFisher Duo Prime protocols for a particular MagMAX kit is the relevant MagMAX kit product page. The Bindlt script protocol (.bdz) files can be found under the Documents>Product literature section.
I see that the KingFisher 24 Deep Well Head has a processing volume of 5 mL. Does that mean that I can process up to 5 mL of my sample?
No, the processing volume refers to the maximum volume that can be held in the 24 Deep Well plates. This incorporates the volume of binding buffers and beads, so when scaling up reaction volumes, it is important to make sure that the total volume does not exceed 5 mL.
My KingFisher Flex run was stopped due to an error message. Can I start the protocol from where it stopped?
No, once a protocol was stopped, it will restart from the beginning. The extraction procedure can either be finished manually by using a magnetic stand, or a modified version of the original KingFisher Flex script would have to be created that starts off at the point where the run was stopped.
