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Thermo Scientific™
Pierce™ Silver Stain Kit
The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels,詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 24612 | 1 L |
製品番号（カタログ番号） 24612
価格（JPY）お問い合わせください ›
44,500
Each
数量:
1 L
The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.
Features:
- Sensitivity—detect proteins at 0.25 ng
- Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
- Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
- Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery
Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
検出位置ゲル内検出
検出法比色法
製品ラインPierce
製品タイプ染色キット
数量1 L
標的分子タンパク質
標識または色素銀
Unit SizeEach
組成および保存条件
対応範囲：20ミニゲル（SDS-PAGE）
• 銀染色増感剤、2 mL
• 銀染色剤、500 mL
• 銀染色エンハンサー、25 mL
• 銀染色現像液、500 mL
室温で保管
• 銀染色増感剤、2 mL
• 銀染色剤、500 mL
• 銀染色エンハンサー、25 mL
• 銀染色現像液、500 mL
室温で保管