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Fetal Bovine Serum, dialyzed, US origin
Fetal Bovine Serum, dialyzed, US origin
Citations & References (2)
Gibco™

Fetal Bovine Serum, dialyzed, US origin

Gibco™ウシ胎児血清は、基礎細胞培養、特殊研究、および特異的アッセイに優れた価値を提供し、研究ニーズや予算要件を満たすことができる、一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ることができます血清カテゴリー：特殊製品原産国：米国。エンドトキシン濃度：≤ 50 EU/mL詳細を見る
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製品番号（カタログ番号）数量フォーマット
26400044500 mLボトル
A338200150 mLOne Shot
製品番号（カタログ番号） 26400044
価格（JPY）
-
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数量:
500 mL
フォーマット:
ボトル

Gibco™ウシ胎児血清は、基礎細胞培養、特殊研究、および特異的アッセイに優れた価値を提供し、研究ニーズや予算要件を満たすことができる、一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ることができます

血清カテゴリー：特殊製品
原産国：米国。
エンドトキシン濃度：≤ 50 EU/mL（仕様の確認と記録済み、通常濃度≤ 10 EU/mL）
ヘモグロビン濃度：≤ 25 mg/dL

研究用途にのみ使用できます
仕様
出荷条件凍結
種畜牛/ウシ
Age胎児
原産国米国
形状液体
フォーマットボトル
製品タイプウシ胎児血清
数量500 mL
Serum Treatment透析済み
Unit SizeEach
組成および保存条件
保存条件：≤-10°C
輸送条件: 凍結

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are proteins removed from Fetal Bovine Serum, dialyzed (Cat. No. 26400044, A3382001)?

The raw serum is processed through a dialysis system that has a 10 kDa cutoff. Therefore, many proteins, peptides, as well as some hormones, glucose, amino acids, and other exogenous materials are removed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, dialyzed, US origin FAQs

引用および参考文献 (2)

引用および参考文献
Abstract
Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitro.
Authors:Courtemanche C, Elson-Schwab I, Mashiyama ST, Kerry N, Ames BN,
Journal:J Immunol
PubMed ID:15322179
'Folate is required for one-carbon transfer reactions and the formation of purines and pyrimidines for DNA and RNA synthesis. Deficiency of folate can lead to many clinical abnormalities, including macrocytic anemia, cardiovascular diseases, birth defects, and carcinogenesis. The nucleotide imbalance due to folate deficiency causes cell cycle arrest in the ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors: Benbow U; Butticè G; Nagase H; Kurkinen M;
Journal:J Biol Chem
PubMed ID:8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More