CaptureSelect™ Bovine IgA Affinity Matrix

CaptureSelect Bovine IgA Affinity Matrix has been designed specifically for the purification of bovine IgA from whey.

Features of this affinity matrix include:

• Binds monomeric, dimeric, and secretory bovine IgA
• One-step purification from whey
• Excellent scalability

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect Bovine IgA affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: ∼10 g/L resin
• Elution conditions: 0.1 M citric acid pH 3.0 or 0.1M glycine pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol