Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
Citations & References (3)
Gibco™

Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

GlutaMAX™-I培地。L-グルタミンの代替となるGlutaMAX™-Iが同じモル数含まれています。詳細を見る
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製品番号（カタログ番号）数量
31765035500 mL
3176509210 x 500 mL
製品番号（カタログ番号） 31765035
価格（JPY）
4,600
Each
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数量:
500 mL
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GlutaMAX™-I培地。

L-グルタミンの代替となるGlutaMAX™-Iが同じモル数含まれています。
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
仕様
細胞株CHO, COS-7, and rat prostate epithelial cells
細胞タイプPrimary Rat Astrocytes
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco, GlutaMAX
製品タイプHam's F-12 Nutrient Mixture
数量500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありGlutaMAX, Phenol Red, Sodium Pyruvate
添加剤なしNo HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement, 500 mL FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.
Authors: Kuroda Taruho S; Fukuda Mitsunori; Ariga Hiroyoshi; Mikoshiba Katsuhiko;
Journal:J Biol Chem
PubMed ID:11773082
rab27A, which encodes a small GTP-binding protein, was recently identified as a gene in which mutations caused human hemophagocytic syndrome (Griscelli syndrome) and ashen mice, which exhibit defects in melanosome transport as well as in regulated granule exocytosis in cytotoxic T lymphocytes. However, little is known about the molecular mechanism ... More
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
Authors: de Diego Iñaki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;
Journal:J Biol Chem
PubMed ID:12070178
Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More
Functional differences of tau isoforms containing 3 or 4 C-terminal repeat regions and the influence of oxidative stress.
Authors: Utton M A; Gibb G M; Burdett I D; Anderton B H; Vandecandelaere A;
Journal:J Biol Chem
PubMed ID:11438517
We report functional differences between tau isoforms with 3 or 4 C-terminal repeats and a difference in susceptibility to oxidative conditions, with respect to the regulation of microtubule dynamics in vitro and tau-microtubule binding in cultured cells. In the presence of dithiothreitol in vitro, a 3-repeat tau isoform promotes microtubule ... More