For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
仕様
細胞株CHO, COS-7, and rat prostate epithelial cells
細胞タイプPrimary Rat Astrocytes
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco, GlutaMAX
製品タイプHam's F-12 Nutrient Mixture
数量10 x 500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありGlutaMAX, Phenol Red, Sodium Pyruvate
添加剤なしNo HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
よくあるご質問(FAQ)
How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.
Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?
In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.
How can I remove mycoplasma contamination from my cell culture medium?
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.