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Thermo Scientific™
Pierce™ ECL Plus Western Blotting Substrate
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray詳細を見る
製品番号（カタログ番号） 32132
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55,200
Each
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
仕様
コンジュゲートHRP
概要Pierce ECL Plus Western Blotting Substrate
キット内容Detection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
感度Low picogram to mid-femtogram
対応可能対象10 mini-gel blots, 1000 cm2 of membrane
検出法Chemiluminescence, Fluorescence
使用対象（アプリケーション）Protein Assay and Analysis
形状Liquid
Membrane CompatibilityNitrocellulose, PVDF
製品ラインPierce
製品タイプ基質
数量100 mL Kit
Unit SizeEach
組成および保存条件
Upon receipt store at 4°C. Product shipped on ice.