MEM α, GlutaMAX™ Supplement, no nucleosides

Citations & References (1)

Gibco™

MEM α, GlutaMAX™ Supplement, no nucleosides

Name: MEM α, GlutaMAX™ Supplement, no nucleosides
SKU: 32561102

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative詳細を見る

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製品番号（カタログ番号）	数量
32561102	10 x 500 mL
32561037	500 mL

2 オプション

製品番号（カタログ番号） 32561102

価格（JPY）

42,000

Each

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数量:

10 x 500 mL

Customize this product

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With	Without
• Phenol Red	• Ribonucleosides
• GlutaMAX™	• Deoxyribonucleosides

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco™ MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

細胞タイプPrimary Rat Astrocytes

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco, GlutaMAX

製品タイプMEM α (Minimum Essential Medium α)

数量10 x 500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありLow Glucose, Phenol Red, Sodium Pyruvate

添加剤なしNo HEPES, No Ribonucleosides, No Deoxyribonucleosides

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM α, GlutaMAX™ Supplement, no nucleosides, 10 x 500 mL FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries.

Authors:Woods DC, Tilly JL

Journal:Nat Protoc

PubMed ID:23598447

Accruing evidence indicates that production of new oocytes (oogenesis) and their enclosure by somatic cells (folliculogenesis) are processes not limited to the perinatal period in mammals. Endpoints ranging from oocyte counts to genetic lineage tracing and transplantation experiments support a paradigm shift in reproductive biology involving active renewal of oocyte-containing ... More