SuperSignal™ West Dura Extended Duration Substrate
SuperSignal™ West Dura Extended Duration Substrate
Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Thermo Scientific SuperSignal West Dura Extended Duration Substrateは、発光ベースのEnhanced chemiluminescence詳細を見る
製品番号(カタログ番号)数量
34075100 mLキット
34076200 mLキット
3707120 mLキット
製品番号(カタログ番号) 34075
価格(JPY)
89,200
Each
お問い合わせください ›
数量:
100 mLキット
一括またはカスタム形式をリクエストする
Thermo Scientific SuperSignal West Dura Extended Duration Substrateは、発光ベースのEnhanced chemiluminescence(ECL)西洋ワサビペルオキシダーゼ(HRP)基質であり、安定した光出力をもつ定量的ウェスタンブロッティングに最適であり、ウェスタンブロット分析におけるミッドフェムトグラムレベルの検出に有用です。

SuperSignal West Dura Extended Duration Substrateの特性:
高感度: 中フェムトグラムレベルの検出
安定性:溶液の効果は24時間持続し、キットは室温で1年間安定
適合性:ニトロセルロース膜およびPVDF膜に対応
シグナル持続時間:24時間
一次抗体の推奨濃度:希釈比1:1,000~1:50,000(0.02~1.0 µg/mL)
二次抗体の推奨濃度:希釈比1:50,000~1:250,000(4~20 ng/mL)

当社のウェスタンブロッティング用化学発光基質の比較と詳細を見る ›

HRP用SuperSignal West Dura Extended Duration Substrateは高い感度とシグナルの長時間持続を実現するように最適化されているため、冷却CCDカメラ検出システムに最適です。シグナル強度が30~60分で検出不可能なレベルにまで減衰してしまう他の基質と異なり、SuperSignal West Dura化学発光基質で生成されたシグナルは24時間安定しているため、複数のフィルムまたはカメラによる露光が可能です。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
キット内容ルミノール/エンハンサー、50 mL, 安定過酸化物バッファー、50 mL
推奨抗体濃度二次:1:50
感度中程のフェムトグラム
シグナル持続時間24時間
対応可能対象12 Mini-Gel Blots, 1000 sq. cm of Membrane
検出法化学発光
使用対象(アプリケーション)Western Blot
形状液体
Membrane CompatibilityNitrocellulose, PVDF
製品ラインSuperSignal
製品タイプ基質
数量100 mLキット
Substrate西洋ワサビペルオキシダーゼ
Unit SizeEach
組成および保存条件
対応範囲:12ミニゲルブロット;1000 cm2相当のメンブレン
• ルミノール/エンハンサー、50 mL
• 安定した過酸化物バッファー、50 mL

室温で保存。

よくあるご質問(FAQ)

What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Dura Extended Duration Substrate?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.