HRP用SuperSignal West Dura Extended Duration Substrateは高い感度とシグナルの長時間持続を実現するように最適化されているため、冷却CCDカメラ検出システムに最適です。シグナル強度が30~60分で検出不可能なレベルにまで減衰してしまう他の基質と異なり、SuperSignal West Dura化学発光基質で生成されたシグナルは24時間安定しているため、複数のフィルムまたはカメラによる露光が可能です。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
キット内容ルミノール/エンハンサー、50 mL, 安定過酸化物バッファー、50 mL
推奨抗体濃度二次:1:50
感度中程のフェムトグラム
シグナル持続時間24時間
対応可能対象12 Mini-Gel Blots, 1000 sq. cm of Membrane
検出法化学発光
使用対象(アプリケーション)Western Blot
形状液体
Membrane CompatibilityNitrocellulose, PVDF
製品ラインSuperSignal
製品タイプ基質
数量100 mLキット
Substrate西洋ワサビペルオキシダーゼ
Unit SizeEach
組成および保存条件
対応範囲:12ミニゲルブロット;1000 cm2相当のメンブレン • ルミノール/エンハンサー、50 mL • 安定した過酸化物バッファー、50 mL
室温で保存。
よくあるご質問(FAQ)
What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?
Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).
Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?
The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.
Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?
Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.