HRP用SuperSignal West Dura Extended Duration Substrateは高い感度とシグナルの長時間持続を実現するように最適化されているため、冷却CCDカメラ検出システムに最適です。シグナル強度が30~60分で検出不可能なレベルにまで減衰してしまう他の基質と異なり、SuperSignal West Dura化学発光基質で生成されたシグナルは24時間安定しているため、複数のフィルムまたはカメラによる露光が可能です。
対応範囲:25ミニゲルブロット;2000 cm2相当のメンブレン • ルミノール/エンハンサー、100 mL • 安定した過酸化物バッファー、100 mL
室温で保存。
よくあるご質問(FAQ)
What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?
Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).
Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?
The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.
Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?
Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.