SuperSignal™ West Femto Maximum Sensitivity Substrate
SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrateは、西洋ワサビペルオキシダーゼ(HRP詳細を見る
製品番号(カタログ番号)数量
34096200 mLキット
3409420 mLキット
34095100 mLキット
製品番号(カタログ番号) 34096
価格(JPY)
173,800
Each
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数量:
200 mLキット
一括またはカスタム形式をリクエストする
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrateは、西洋ワサビペルオキシダーゼ(HRP)酵素によるウェスタンブロッティングで低フェムトグラムレベルのタンパク質を検出するための、超高感度の高度化学発光(ECL)基質です。

SuperSignal West Femto Maximum Sensitivity Substrateの特性:
高感度: 低フェムトグラム
安定性:溶液の効果は8時間持続し、キットは4℃で1年間安定
適合性:ニトロセルロース膜およびPVDF膜に対応
シグナル持続時間:最長8時間
一次抗体の推奨濃度:希釈比1:5,000~1:100,000(10~200 ng/mL)
二次抗体の推奨濃度:希釈比1:100,000~1:500,000(2~10 ng/mL)

当社のウェスタンブロッティング用化学発光基質の比較と詳細を見る›
使用可能なすべてのHRP基質を見る›

SuperSignal West Femto Maximum Sensitivity Substrateは、ウェスタンブロット解析による低フェムトグラムレベルの検出を実現するよう設計されています。SuperSignal West Femto基質は、最適な抗体濃度およびブロッキングバッファーと組み合わせることで、標準的なECL基質では確認できない微量の標的タンパク質の検出を可能にします。

研究用途にのみご使用ください。診断目的には使用できません。
仕様
コンジュゲートHRP(西洋ワサビペルオキシダーゼ)
キット内容安定過酸化物バッファー、100 mL, ルミノール/エンハンサー、100 mL
推奨抗体濃度二次:1:100~500,000 (2~10 ng/mL)、一次:1:5,000(10~200 ng/mL)
感度低~中フェムトグラム
シグナル持続時間6~8時間
対応可能対象25 mini-gel blots, 2000 sq. cm of membrane
検出法化学発光
使用対象(アプリケーション)Western Blot
形状液体
Membrane CompatibilityNitrocellulose, PVDF
製品ラインSuperSignal
製品タイプ基質
数量200 mLキット
Substrate西洋ワサビペルオキシダーゼ
Unit SizeEach
組成および保存条件
対応範囲:25ミニゲルブロット、2000 cm2相当のメンブレン
• ルミノール/エンハンサー、100 mL
• 安定した過酸化物バッファー、100 mL

試薬は4℃で保存。

よくあるご質問(FAQ)

What types of membrane work best with SuperSignal West Femto Maximum Sensitivity Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Femto Maximum Sensitivity Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Femto Maximum Sensitivity Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Femto Maximum Sensitivity Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Femto Maximum Sensitivity Substrate ?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

引用および参考文献 (1)

引用および参考文献
Abstract
Natural IgG autoantibodies are abundant and ubiquitous in human sera, and their number is influenced by age, gender, and disease.
Authors:Nagele EP, Han M, Acharya NK, DeMarshall C, Kosciuk MC, Nagele RG
Journal:PLoS One
PubMed ID:23589757
The presence of self-reactive IgG autoantibodies in human sera is largely thought to represent a breakdown in central tolerance and is typically regarded as a harbinger of autoimmune pathology. In the present study, immune-response profiling of human serum from 166 individuals via human protein microarrays demonstrates that IgG autoantibodies are ... More