TaqMan™ β-Actin Detection Reagents
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Applied Biosystems™

TaqMan™ β-Actin Detection Reagents

Applied Biosystems™ TaqMan™ β-actin provides the necessary components for using β-actin as a normalization control in TaqMan™ real-time PCR applications詳細を見る
製品番号(カタログ番号)数量
401846100 reactions
製品番号(カタログ番号) 401846
価格(JPY)
64,100
Each
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数量:
100 reactions
Applied Biosystems™ TaqMan™ β-actin provides the necessary components for using β-actin as a normalization control in TaqMan™ real-time PCR applications employing human nucleic acid. Either DNA or RNA can be used as a target template.

• Pre-designed primers and FAM-labeled TaqMan™ probe eliminate assay design

• Rapid assay development guidelines can minimize optimization time

• Separate components provide flexibility in assay set-up

Notes on Use

The TaqMan™ β-actin Detection Reagent kit can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

Notice to Purchaser
1. Disclaimer of License: These products may be useful in the Polymerase Chain Reaction (“PCR”) methods covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. However, no license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.

TaqMan™ probes are covered by US Patent 5,723,591 and foreign counterparts and patents pending owned by PE Corporation (NY), and may be covered by US Patents 5,801,155 and 6,084,102 and foreign counterparts licensed to Applied Biosystems™

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
コントロールテンプレートHuman Control DNA
フォーマットTube
遺伝子記号ACTB
内部プローブの変更TAMRA™ Quencher (3'), FAM (5')
反応数反応100回分
製品ラインTaqMan
製品タイプReal Time PCR Reagents
数量100 reactions
出荷条件ドライアイス
Human
対応可能対象100反応
ターゲットβ-actin
標識または色素FAM
PCR法1-step RT-qPCR
Unit SizeEach
組成および保存条件
Control Template(s)

よくあるご質問(FAQ)

TaqMan® Beta-actin Detection Reagents(Cat. No. 401846)を用いてマウスのgDNAを増幅することはできますか?

このキットはヒトのDNA配列情報を利用して作成されておりますので他の動物種のDNA測定用にご利用いただくことは保証されておりません。
TaqMan® Gene Expression Assaysのシリーズで各生物種に対応したキットをご用意しておりますのでそちらをご利用ください。

Can I use the TaqMan β-actin Detection Reagents (Cat. No. 401846) as an endogenous control for my gene expression studies?

The TaqMan β-actin Detection Reagents were designed to amplify genomic DNA, and thus are not recommended for use as an endogenous control for gene expression studies. However, we do offer other TaqMan assay reagents that were specifically designed for that application. Two examples are: Human ACTB (Beta Actin) Endogenous Control, Cat. No. 4310881E (TAMRA probe) or Cat. No. 4326315E (MGB probe).

There are many factors to consider when validating a specific endogenous control. For additional information on designing gene expression assays and controls, please refer to the following documents: "Protocol: TaqMan Human Endogenous Control Plate", and User Bulletin #2: "Relative Quantitation of Gene Expression". You can find copies of these documents on our website by entering the titles in the main Search field.

Can TaqMan Beta-actin Detection Reagents, Cat. No. 401846, be used for both human and mouse genomic DNA?

This particular kit was designed for human DNA. Our current Beta-actin Detection Reagents are designed for human targets only. Please check our online search tools for other Beta actin reagents for human (Hs), mouse (Mm) or rat (Rn) targets.

Where can I find the TaqMan Actin Detection Reagents (P/N 401846) manual?

Complete instructions for the TaqMan B-actin Detection Reagents can be found in the manual titled "TaqMan PCR Reagent Kit with Controls" on the product page.

引用および参考文献 (1)

引用および参考文献
Abstract
In situ detection of starvation-induced autophagy.
Authors:Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM,
Journal:J Histochem Cytochem
PubMed ID:16148314
Autophagy is a regulated bulk degradation process involved in many different human pathologies. Transmission electron microscopy (TEM) is currently the only reliable method for monitoring autophagy in situ. Because TEM is labor intensive, we questioned whether useful marker proteins can be found for unambiguous detection of autophagy in tissue via ... More