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Gibco™

MEM, GlutaMAX™ Supplement

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used詳細を見る
製品番号(カタログ番号)数量
4109009310 x 500 mL
製品番号(カタログ番号) 41090093
価格(JPY)
-
数量:
10 x 500 mL
Customize this product
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• GlutaMAX™ • HEPES
• Phenol Red

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. Gibco™ MEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ MEM product made in our Grand Island facility (41090-101). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞タイプMammalian Cells
濃度1 X
培養環境CO2
浸透圧280 - 320 mOsm/kg
製品ラインGibco, GlutaMAX
製品タイプMinimum Essential Medium (MEM)
数量10 x 500 mL
品質保持期間12 Months
分類Animal Origin-free
Culture TypeMammalian Cell Culture
形状Liquid
無菌性Sterile-filtered
添加剤ありGlutaMAX, Phenol Red, Sodium Bicarbonate
添加剤なしNo HEPES, No Sodium Pyruvate
pH7 to 7.4
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

よくあるご質問(FAQ)

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.