MicroAmp™ Optical Adhesive Film

Please follow the instructions below for the proper application of MicroAmp Optical Adhesive Covers (Cat. Nos. 4311971, 4313663 and 4360954).

The MicroAmp Optical Adhesive Cover uses a pressure-sensitive adhesive backing to adhere the cover to the optical 96- or 384-well plate. It is imperative that you use enough force to activate the pressure-sensitive adhesive to prevent evaporation from the wells.
Please note: Users should not expect the Optical Adhesive Cover to be very sticky to the touch on the side that adheres to the optical plate.

The correct way to apply the cover is as follows:

1. Apply firm pressure when using the Adhesive Seal Applicator (Cat. No. 4333183) to press the cover onto the plate.

2. Run the applicator firmly from left to right several times. Run the applicator up and down several times.

3. Run the edge of the applicator around the border (following the red line outside the wells) of the cover.

4. Repeat the left to right and up and down motions to assure a good final seal.

5. Inspect each well to make sure that each well edges are imprinted on the optical adhesive cover.
Please Note: Using the Applicator's edge to seal between the wells is NOT recommended. It may actually pull the seal off the wells.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

There are many of our reagents and plastic consumables that can be used on the Applied Biosystems 7900HT Fast Real-Time PCR System. Here are a few examples to help you get started:
Cat. No. 4313663, MicroAmp Optical Adhesive Film Kit
Cat. No. 4311971, 4360954, MicroAmp Optical Adhesive Film
Cat. No. 4323032, MicroAmp Optical 8-Cap Strip
Cat. No. 4309849; 4326270, MicroAmp Optical 384-Well Reaction Plate with Barcode
Cat. No. 4306737; 4326659; 4314320, MicroAmp Optical 96-Well Reaction Plate with Barcode
Cat. No. 4346906, MicroAmp Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL
Cat. No. 4312063, Splash Free 96-Well Base

For more comprehensive lists of compatible plastics, enter "Real-Time PCR Plastics" in the main Search field on our website and follow the links.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

No. The Optical Adhesive Film is recommended for use only with the MicroAmp Optical 96-well Reaction Plate (with or without barcode) and MicroAmp 384-well Optical Reaction Plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The MicroAmp Optical Adhesive Covers (P/N 4311971) have a higher optical efficiency than the MicroAmp Optical Caps, (8 caps/strip) (P/N 4323032). They can be used instead of the MicroAmp Optical Caps only when using the MicroAmp 96-Well Reaction Plate with Barcode (P/N 4306737, 4309849) or the MicroAmp Optical 96-Well Reaction Plate (P/N N801-0560). The Optical Adhesive Covers cannot be used with the MicroAmp Optical Tubes.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.