TaqMan™ MGB Probe

Applied Biosystems™

TaqMan™ MGB Probe

Name: TaqMan™ MGB Probe
SKU: 4316034

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.

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製品番号（カタログ番号）	5'プライマー修飾	数量
4316034	FAM, NED, TET, VIC	6,000 pmol Scale
A40005000	ABY	6,000 pmol
A40005001	ABY	20,000 pmol
A40005002	ABY	50,000 pmol
A40005003	JUN	6,000 pmol
A40005004	JUN	20,000 pmol
A40005005	JUN	50,000 pmol
A40005006	Cyanine 5	6,000 pmol
A40005007	Cyanine 5	20,000 pmol
A40005008	Cyanine 5	50,000 pmol
4316032	FAM, NED, TET, VIC	50,000 pmol Scale
4316033	FAM, NED, TET, VIC	20,000 pmol Scale

12 オプション

製品番号（カタログ番号） 4316034

価格（JPY）

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5'プライマー修飾:

FAM, NED, TET, VIC

数量:

6,000 pmol Scale

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB). Choose from FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED as reporters. Probes are HPLC-purified.

Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED reporter dyes.

Shorter, more specific probes

TaqMan MGB probes include a MGB moiety at the 3' end that increases the melting temperature (Tm) of the probe and stabilizes probe/target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.

Nonfluorescent quencher (NFQ) maximizes sensitivity

TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.

Multiplexing

The availability of MGB probes for up to five channels allows for higher-order multiplexing, enabling detection of more targets from the same samples.

Guaranteed yield

The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time

4–10 days in North America, 5-10 days in Europe

We also offer:

Applied Biosystems custom primers and other types of TaqMan probes.
TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.

研究用途にのみご使用ください。診断目的には使用できません。

仕様

3'プライマー修飾MGB-NFQ

5'プライマー修飾FAM, NED, TET, VIC

使用対象 (装置)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System

フォーマットTube

グリーン機能Sustainable packaging

内部プローブの変更None

製品ラインTaqMan

製品タイプTaqMan Probe

精製方法HPLC

純度または品質グレードHPLC

数量6,000 pmol Scale

出荷条件Room Temperature

使用対象（アプリケーション）Real Time PCR (qPCR)

形状液体

標識または色素FAM, VIC, NED, TET

Unit SizeEach

よくあるご質問（FAQ）

TaqMan® probeの代わりに蛍光プライマーを使うことはできますか

蛍光プライマーはTaqMan®プローブの代わりには利用できません。
リアルタイムPCRで蛍光プライマーを利用することはサポートしていません。

I would like to calibrate my Real-Time PCR instrument with a custom dye, which buffer should I use?

Please ask your dye manufacturer for recommendations. If there is no specific recommendation available, try TE Buffer (pH 8.0) to start.

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

I have purchased custom oligos (primers and probes) and I want to make a 20x working stock for a TaqMan assay, similar to the pre-designed assays. How do I dilute and mix my oligos to make a 20x working stock?

Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.

In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.

View all TaqMan™ MGB Probe FAQs