The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT-rich regions • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
仕様
概要
Cycle Sequencing Kit, 1000 rxns, Includes 10 Tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21) Primer, pGEM™-3Zf(+) double-stranded DNA Control Template, 1 x 12mL tube of 5X Sequencing Buffer (may be stored at 4°C)
フォーマット
12-well Strips, 8-well Strip, 96- or 384-well Plate
Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
内容
10 Tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21) Primer, pGEM™-3Zf(+) double-stranded DNA Control Template, 1 x 12mL tube of 5X Sequencing Buffer (may be stored at 4°C)
反応数
1000
製品ライン
BigDye™
製品タイプ
Sequencing Buffer
技術
Fluorescent Dye Terminator Sequencing
テンプレート適合性
BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), Single Stranded DNA
組成および保存条件
• 10 tubes of BigDye™ Terminator v3.1 Ready Reaction Mix • M13 (-21) Primer • pGEM™-3Zf(+) double-stranded DNA Control Template • 1 × 12 ml tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.