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推奨事項
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
仕様
概要
Cycle Sequencing Kit, 5000 rxns, Includes 2 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 2 tubes M13 (-21) Primer, 2 tubes pGEM Control DNA, 2 x 28mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
フォーマット
12-well Strips, 8-well Strip, 96- or 384-well Plate
使用対象 (装置)
310 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
使用対象（アプリケーション）
Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
内容
2 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 2 tubes M13 (-21) Primer, 2 tubes pGEM Control DNA, 2 x 28mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
反応数
5000
製品ライン
BigDye™ Terminator, BigDye™
製品タイプ
Sequencing Buffer
技術
Fluorescent Dye Terminator Sequencing
テンプレート適合性
BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA
組成および保存条件
• 2 × 20-ml tubes of BigDye™ Terminator v3.1 Ready Reaction Mix
• 2 tubes M13 (-21) Primer
• 2 tubes pGEM Control DNA
• 2 × 28-ml tubes of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.
• 2 tubes M13 (-21) Primer
• 2 tubes pGEM Control DNA
• 2 × 28-ml tubes of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.
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