Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems

Applied Biosystems 7500 Real-time PCR System または Applied Biosystems 7500 Fast Real-Time PCR System では、MGB-NFQのProbeを使用した場合、以下の蛍光dyeをリポーター蛍光として利用することが可能です [ 注意：　TaqMan Gene Expression Master Mix (multiplex解析にはお薦めのMaster Mixです）またはApplied Biosystemsから提供するTaqMan master mixを利用した場合]:
FAM, VIC, JOE, NED, TAMRA,CY3, ROX, TEXAS RED, CY5 dyes.

duplex（2アッセイ）のmultiplex反応を行う場合、 FAM と VICの組合せ、またはFAM と NED の組合せで反応を行うことをお薦めします。またtriplex（3アッセイ）のmultiplex反応を行う場合、 FAM, VIC, Cy5 の組合せ、またはFAM, VIC,TAMRAの組合せで反応を行うことをお薦めします。蛍光の組合せは、お互いの発光波長がなるべく大きく異なる蛍光を選択します。またTAMRAクエンチャー のProbeを使用する場合、Multiplex反応にNED, TAMRA, CY3 は使用できません。ただしApplied Biosystems Master Mix以外の、レファレンス蛍光としてROXを添加していないMaster Mixを使用した場合、 ROX または TEXAS RED が使用できます。

TET dye is not recommended to be used on the following real-time PCR instrument models: 7000, 7300, 7500, 7500 FAST, StepOne, and StepOnePlus Systems.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The Applied Biosystems 7300 Real-Time PCR System has 4 signal detection filters: Filter A (FAM and SYBR Green), Filter B (VIC and JOE), Filter C (TAMRA, NED and Cy3) and Filter D (ROX and Texas Red). For multiplexing reactions, the reporter dyes from the different assays should be chosen from different filter sets, so they can be compatible. In addition, the reporter dye need to be compatible with the quencher and the passive reference dye that might be present in the reaction. The commonly used combination is FAM and VIC.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

For the 7500 Fast real time PCR systems, the thermal cycling parameters are defaulted to 50°C/2 min for UNG incubation, 95°C/20 sec for fast polymerase activation, 40 cycles of 95°C/3 secs for the dissociation and 60°C/30 seconds for annealing and extensions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Yes. you can choose either standard or fast ramp on the 7500 Fast System for software versions 2x or greater, and a 9600 emulation thermal cycling mode is available for customers who wish to run assays based on adjusted ramp rate. However, you should be aware that we cannot guarantee identical performance to the same modes run on a standard 7500 system block.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.