TaqMan™ MicroRNA Reverse Transcription Kit
TaqMan™ MicroRNA Reverse Transcription Kit
Applied Biosystems™

TaqMan™ MicroRNA Reverse Transcription Kit

The Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan詳細を見る
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製品番号（カタログ番号）対応可能対象
43665971000 Reactions
4366596200 Reactions
製品番号（カタログ番号） 4366597
価格（JPY）
108,000
Each
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対応可能対象:
1000 Reactions

The Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.

This is a highly specific kit that quantitates only mature miRNAs, not precursors and a sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is a fast, simple, and scalable two-step quantitative RT-PCR assay that provides high-quality results in less than three hours.

Note: The amount of RT enzyme included in the kit is sufficient for single tubes but not for arrays.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
フィデリティ（vs. Taq）10 X
最終産物タイプcDNA
反応数1000 Reactions
最適反応温度42°C
製品ラインTaqMan
製品タイプMicroRNA Reverse Transcription Kit
数量1,000 reactions
反応形態別のコンポーネント
逆転写酵素MultiScribe™
サンプルタイプRNA
出荷条件ドライアイス
サイズ（最終製品）200 bp or less
対応可能対象1000 Reactions
濃度10X
検出法Primer-probe
使用対象（アプリケーション）miRNA analysis
GC-Rich PCR Performance高
PCR法2ステップRT-qPCR
反応速度高速
Unit SizeEach
組成および保存条件
Contains 10X RT buffer, dNTP mix w/dTTp (100 mM total), RNase inhibitor (20 U/μL), and MultiScribe™ RT enzyme (50 U/μL) for 1000 reactions. Store at -15 to -25°C.

よくあるご質問（FAQ）

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Is it possible to combine several miRNA RT primers together for reverse transcription, and if so, how?

Yes. If you are using individual TaqMan MicroRNA Assays, you can prepare your own RT and PreAmp pools. Refer to Protocol for Creating Custom RT and Preamplification Pools using TaqMan MicroRNA Assays (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4465407_CustomRT-preamp_UG.pdf) for detailed information. Although this protocol has been tested by our development group, we recommend that you validate the performance of the particular pool that you are interested in working with. At a minimum, we recommend running a No Template Control (NTC). The NTC is crucial to identify any primer interactions that may increase the background.

Why does the TaqMan MicroRNA Reverse Transcription Kit (4366597) protocol have an initial 16 degree C step before the reverse transcription step at 42 degrees C?

The 30 min incubation at 16°C allows the RT primer to anneal to miRNA template properly. See the complete protocol at this link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of cDNA or range do we recommend for miRNA assays when using the TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)?

1-10 ng of total RNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all TaqMan™ MicroRNA Reverse Transcription Kit, 1,000 reactions FAQs