High-Capacity cDNA Reverse Transcription Kit

Citations & References (1122)

Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit

Name: High-Capacity cDNA Reverse Transcription Kit
SKU: 4368813

High Capacity cDNA Reverse Transcription Kitには、単一の20 µLの反応で最大2 µgの全RNAを一本鎖cDNAに変換するために必要な、すべての試薬が含まれています詳細を見る

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製品番号（カタログ番号）	内容	反応数
4368813	Kit only	1000反応
4368814	Kit only	200反応
4374966	Kit with Rnase Inhibitor	200反応
4374967	Kit with Rnase Inhibitor	1000反応

4 オプション

製品番号（カタログ番号） 4368813

価格（JPY）

215,000

Each

お問い合わせください ›

内容:

Kit only

反応数:

1000反応

High Capacity cDNA Reverse Transcription Kitには、単一の20 µLの反応で最大2 µgの全RNAを一本鎖cDNAに変換するために必要な、すべての試薬が含まれています。高容量cDNA逆転写を使用すると、0.02～2 µgの全RNAから高品質の一本鎖cDNAが得られます。単一の反応で10 µgのcDNAを生成するために、反応を100 µLまで拡張できます。ダウンストリームアプリケーションは、リアルタイムPCR、標準PCR、マイクロアレイなどです。このキットは、cDNAアーカイブの生成に最適です。

このキットの特長は以下のとおりです。

•リアルタイムPCRのためのターゲットの線形増幅
• 他のcDNA合成キットよりも高い収量と精度が数分の一のコストで得られる
• ダイナミックレンジが他のキットより10倍高い

さまざまなテンプレートで広範に試験済み
ランダムプライマーを使用することで、すべてのRNA種の定量的ファーストストランド合成を実現できます。このキットは、GCおよびAUリッチターゲットや低レベルで発現したRNAなど、さまざまなRNAテンプレートで広範に試験されています。また、cDNAのin vitro転写からcRNAを効率的に生成することもできます。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

最終産物タイプcDNA（ファーストストランド）

フォーマットキット

内容Kit only

反応数1000反応

数量1 Ea.

反応形態分離コンポーネント

試薬タイプ逆転写反応試薬

逆転写酵素MultiScribe

リボヌクレアーゼH活性可

出荷条件ドライアイス

サイズ（最終製品）7 kb

原料RNA

技術Reverse Transcription

濃度50 U/μL

使用対象（アプリケーション）Real Time PCR (qPCR), RT-PCR

反応速度スタンダード

Unit SizeEach

組成および保存条件

• 10X RT Buffer (2 x 1 mL)
• 10X RT Random Primers (2 x 1 mL)
• 25X dNTP Mix (1.0 mL at 100 mM)
• MultiScribe Reverse Transcriptase (1.0 mL at 50 U/μL)

Store at –15 to –25°C.

よくあるご質問（FAQ）

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the concentration of the random primers in the High-Capacity cDNA Reverse Transcription Kit (Cat. no. 4368813)?

The concentration of the "random primers" in the High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813) is 25 µM. We recommend that you use these primers at a 1X concentration (2.5 µM) in reverse transcription reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?

It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).

引用および参考文献 (1122)

引用および参考文献

Abstract

Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.

Authors:Vigetti D,Viola M,Karousou E,Rizzi M,Moretto P,Genasetti A,Clerici M,Hascall VC,De Luca G,Passi A

Journal:The Journal of biological chemistry

PubMed ID:18077444

Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.

Authors:Wong H,Lehman-McKeeman LD,Grubb MF,Grossman SJ,Bhaskaran VM,Solon EG,Shen HS,Gerson RJ,Car BD,Zhao B,Gemzik B

Journal:Toxicological sciences : an official journal of the Society of Toxicology

PubMed ID:15673846

4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell ... More

Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.

Authors:Lawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K

Journal:J Biol Chem

PubMed ID:21059644

Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet alpha cells and beta cells express tumor necrosis factor-alpha (TNF-alpha) and other ... More

TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.

Authors:Winbanks CE, Wang B, Beyer C, Koh P, White L, Kantharidis P, Gregorevic P

Journal:J Biol Chem

PubMed ID:21324893

MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of ... More

Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.

Authors:Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW

Journal:J Exp Med

PubMed ID:19652016

The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance of bacteria penetrating the lining of the bowel and predispose to granuloma formation and chronicity. We tested this hypothesis in human subjects by monitoring responses to ... More

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