Fast SYBR™ Green Master Mix
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Applied Biosystems™

Fast SYBR™ Green Master Mix

Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for詳細を見る
製品番号(カタログ番号)数量
43856101 x 1 mL
43856121 x 5 mL
43856162 x 5 mL
43856175 x 5 mL
438561810 x 5 mL
43856141 x 50 mL
製品番号(カタログ番号) 4385610
価格(JPY)
12,600
Each
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数量:
1 x 1 mL
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7500高速システム、7900HT高速システム、StepOne™、高速モード、StepOnePlus™、高速モード
フォーマットTube
反応数反応100回分
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
製品ラインSYBR
製品タイプReal Time PCR SYBR Master Mix
純度または品質グレード高(超高純度)
数量1 x 1 mL
サンプルタイプDNA (Genomic), cDNA
出荷条件ドライアイス
対応可能対象100 Reactions of 20 μL
容量1 mL
濃度2X
検出法SYBR
使用対象(アプリケーション)遺伝子発現解析
GC-Rich PCR Performance
標識または色素SYBR Green
PCR法qPCR
反応速度高速
Unit SizeEach
組成および保存条件
1 x 1 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. 100 reactions in a 20 μL total volume. Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問(FAQ)

Is there a SYBR Green Fast Master Mix currently available?

Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.

引用および参考文献 (1)

引用および参考文献
Abstract
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More