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Applied Biosystems™
Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
製品番号（カタログ番号） 4389986
価格（JPY）お問い合わせください ›
106,200
Each
反応数:
500反応
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:
- AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
- ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
- An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
- RNase inhibitor, to prevent degradation of RNA templates
- A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
反応数500反応
受動的参照色素ROX（プレミックス）
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインRNA-to-CT, SYBR
製品タイプOne-Step qRT-PCR Kit
純度または品質グレード高（超高純度）
数量500 Reactions
逆転写酵素ArrayScript™ UP
ランタイムStandard
サンプルタイプRNA
出荷条件Wet Ice
対応可能対象500 Reactions at 20 μL
検出法SYBR
使用対象（アプリケーション）遺伝子発現解析
標識または色素SYBR Green
PCR法1-step RT-qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Sufficient for 500 reactions at 20 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).