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Applied Biosystems™
TaqMan™ RNA-to-CT™ 1-Step Kit
The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is詳細を見る
製品番号（カタログ番号） 4392938
価格（JPY）お問い合わせください ›
116,300
Each
反応数:
200反応
The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems comprehensive library of TaqMan Gene Expression assays. The kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.
Features of the TaqMan RNA-to-CT 1-Step Kit include:
• Accurate across a wide dynamic range for dependable performance
• Validated with TaqMan Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• 1-Step format means fewer pipetting steps leading to less chances for errors
Efficiency across a broad range of input concentrations
Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities. Figure 1 shows the excellent amplification efficiency across six orders of magnitude. The result is comparable to that of the TaqMan RNA-to-CT 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan One-Step RT-PCR Master Mix Reagents.
High sensitivity at low target concentration
The sensitivity of TaqMan RNA-to-CT 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target*.
*Results are dependent on a variety of factors, including assay design.
Features of the TaqMan RNA-to-CT 1-Step Kit include:
• Accurate across a wide dynamic range for dependable performance
• Validated with TaqMan Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• 1-Step format means fewer pipetting steps leading to less chances for errors
Efficiency across a broad range of input concentrations
Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities. Figure 1 shows the excellent amplification efficiency across six orders of magnitude. The result is comparable to that of the TaqMan RNA-to-CT 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan One-Step RT-PCR Master Mix Reagents.
High sensitivity at low target concentration
The sensitivity of TaqMan RNA-to-CT 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target*.
*Results are dependent on a variety of factors, including assay design.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
反応数200反応
受動的参照色素ROX（プレミックス）
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインRNA-to-CT, TaqMan
製品タイプOne-Step RT-PCR Kit
純度または品質グレード高（超高純度）
数量200 reactions
逆転写酵素ArrayScript™ UP
サンプルタイプRNA
出荷条件Wet Ice
対応可能対象200 Reactions
検出法Primer-probe
使用対象（アプリケーション）遺伝子発現解析
PCR法1-step RT-qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Contains sufficient reagents for 200 reactions at 50 μl reaction volumes. Includes:
• 1 × 5 ml tube of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C.
• 1 × 250 μl tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15°C to -30°C.
• 1 × 5 ml tube of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C.
• 1 × 250 μl tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15°C to -30°C.