TaqMan™ Environmental Master Mix 2.0
TaqMan™ Environmental Master Mix 2.0
実際の製品は異なる場合があります
Applied Biosystems™

TaqMan™ Environmental Master Mix 2.0

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of詳細を見る
製品番号(カタログ番号)反応数
4398021反応400回分
4396838200反応
4398044800 Reactions
製品番号(カタログ番号) 4398021
価格(JPY)
126,300
Each
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反応数:
反応400回分
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.

- Tailored for quantitative, real-time PCR experiments
- Unrivalled sensitivity for challenging applications, low copy number target detection and multiplex PCR
- Includes pathogen detection and viral⁄bacterial load quantification
- Sensitive detection for reliable quantification of abundant and limited genetic targets
- Stable mix for high-throughput handling

Optimized Formulation for Unrivalled Performance

TaqMan™ Environmental Master Mix 2.0 is a convenient 2X mix for target quantification that includes:

- AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure), a highly purified DNA Polymerase for improved detection of bacterial targets. This hot-start enzyme is inactive at room temperature so reactions can be set up on the benchtop. Enzyme is activated during thermal cycling.
- Passive internal reference based on proprietary ROX™ dye for precise data analysis.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
フォーマットBottle
ホットスタート内蔵ホットスタート
反応数反応400回分
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインTaqMan
製品タイプqPCR Master Mix
純度または品質グレード高(超高純度)
数量400 reactions
サンプルタイプEnvironmental Sample, Food
対応可能対象400反応
容量10 mL
濃度2X
検出法Primer-probe
使用対象(アプリケーション)遺伝子発現解析, Genotyping, Pathogen Detection, Virus Detection
PCR法qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
This TaqMan™ Environmental Master Mix 2.0 is a convenient 2X mix in a 10 mL bottle that includes: AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), and a passive internal reference based on proprietary ROX dye for precise data analysis.
Upon receipt, store at -15 to -25°C before first use, protected from light. After first use, store at 2 to 8°C, protected from light.

よくあるご質問(FAQ)

Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.

Is the TaqMan Environmental Master Mix 2.0 (Cat. No. 4398021, 4398044, 4396838) compatible with the QuantStudio instruments?

Yes, it is.

Find additional tips, troubleshooting help, and resources within our Gene Expression Analysis & Genotyping Support Center.

I have run out of Environmental Master Mix v2.0 from the Ion 16S Metagenomics Kit (Cat. No. A26216). Do you offer this as a standalone item?

Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

Is there a mastermix that I can use with fecal samples?

For fecal samples, we recommend a master mix that is very tolerant of inhibitors, such as our TaqMan Environmental Master Mix 2.0. Please refer to the following paper: https://assets.thermofisher.com/TFS-Assets/CMD/posters/cms_089094.pdf