Megaplex™ RT Primers, Human Pool A v2.1

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis詳細を見る

製品番号（カタログ番号）	数量
4399966	50 reactions

製品番号（カタログ番号） 4399966

価格（JPY）

128,500

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50 reactions

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A v2.1 is intended for use with the TaqMan® Array, Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

研究用途にのみご使用ください。診断目的には使用できません。

グリーン機能Sustainable packaging

反応数50 Reactions

プライマーRT Primers

製品ラインMegaplex, TaqMan

製品タイプRT Primer

数量50 reactions

出荷条件Room Temperature

種Human

対応可能対象50 Reactions

使用対象（アプリケーション）miRNA analysis

GC-Rich PCR PerformanceHigh

PCR法qPCR

Unit SizeEach

組成および保存条件

Store at -20°C.

よくあるご質問（FAQ）

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all Megaplex™ RT Primers, Human Pool A v2.1 FAQs