TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
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Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)

The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one詳細を見る
製品番号(カタログ番号)数量
44033131 kit
製品番号(カタログ番号) 4403313
価格(JPY)
52,000
Each
お問い合わせください ›
数量:
1 kit
The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 μL/ reaction and 400 PCR reactions at 5 μL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode
ジェノタイピングターゲットSNPs (Unknown or Numerous), SNPs (Known)
ホットスタート内蔵ホットスタート
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq DNA Polymerase
製品ラインSample-to-SNP, TaqMan
製品タイプSample to SNP Kit
純度または品質グレード高(超高純度)
数量1 kit
サンプルタイプBuccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
対応可能対象100反応
技術SNP Genotyping (Real-Time PCR-Based)
容量5 mL
検出法Primer-probe
GC-Rich PCR Performance
PCR法qPCR
反応速度高速または標準
Unit SizeEach
組成および保存条件
Lysis Reagents (2° to 8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C ): 1 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問(FAQ)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".