TaqMan™ OpenArray™ Genotyping Master Mix

This 2X concentrated mix is optimized for SNP genotyping using TaqMan™ OpenArray™ SNP Genotyping Plates.For Research Use Only. Not for詳細を見る

製品番号（カタログ番号）	数量
4404846	1 kit

製品番号（カタログ番号） 4404846

価格（JPY）

59,300

お問い合わせください ›

1 kit

This 2X concentrated mix is optimized for SNP genotyping using TaqMan™ OpenArray™ SNP Genotyping Plates.

For Research Use Only. Not for use in diagnostics procedures.

研究用途にのみご使用ください。診断目的には使用できません。

フィデリティ（vs. Taq）2 X

使用対象 (装置)OpenArray™

ジェノタイピングターゲットDrug Metabolizing Enzymes, SNPs (Known)

ホットスタート内蔵ホットスタート

受動的参照色素ROX（プレミックス）

ポリメラーゼAmpliTaq Gold DNA Polymerase

製品ラインOpenArray, TaqMan

製品タイプGenotyping Master Mix

数量1 kit

サンプルタイプDNA

出荷条件Wet Ice

技術SNP Genotyping (Real-Time PCR-Based)

検出法Primer-probe

使用対象（アプリケーション）Genotyping

PCR法qPCR

反応速度スタンダード

Unit SizeEach

組成および保存条件

Includes TaqMan™ OpenArray™ Genotyping Master Mix, with AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs, and optimized mix components. This pack contains one 5 mL bottle, sufficient for 32 plates. Store at 2–8°C.

よくあるご質問（FAQ）

What are the cycling conditions for OpenArray off-line cycling on a ProFlex PCR System?

These instructions may be found on page 79 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf).

Reaction volume: 33 nL
Cover temperature: 70°C

94°C for 600 sec, ramp rate 1.3

50 cycles of:
99°C for 30 seconds, ramp rate 1.3
54°C for 21 seconds, ramp rate 1.1
57°C for 90 seconds, ramp rate 0.1

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the cycling conditions for OpenArray off-line cycling on a GeneAmp PCR System 9700?

These instructions may be found on page 5 of the Dual Flat Block GeneAmp PCR System 9700 User Guide (https://tools.thermofisher.com/content/sfs/manuals/cms_065075.pdf).

93°C for 10 min, ramp rate 100%

50 cycles of:
95°C for 45 sec, 84% ramp rate
94°C for 13 sec, 100% ramp rate
53°C for 2 min 14 sec, 44% ramp rate

25°C for 2 min, 100% ramp rate

Optional: Add a post-PCR overnight hold as shown below:
4°C for 99 min 59 sec, 100% ramp rate

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What additional accessories do I need to perform off-line cycling for QuantStudio OpenArray plates?

You need an OpenArray Plate Adaptor, Cat. No. 4472306. You do not need a compression pad. This part number works with both the GeneAmp PCR System 9700 and the ProFlex PCR System.
Please note that this part number does not have a product page on our website. To order, please call our customer service team at 800-955-6288, option 1, option 1.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I get additional throughput for the QuantStudio 12K Flex OpenArray System?

Additional OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the GeneAmp PCR System 9700 and the ProFlex PCR System.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.