MeltDoctor™ HRM Reagent Kit

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution詳細を見る

製品番号（カタログ番号）	数量
4425557	1 kit

製品番号（カタログ番号） 4425557

価格（JPY）

103,600

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1 kit

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp™ dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

研究用途にのみご使用ください。診断目的には使用できません。

使用対象 (装置)7500 Fast System, 7900HT Fast System

ジェノタイピングターゲットSNPs (Unknown or Numerous), Epigenetic Targets

ポリメラーゼAmpliTaq Gold 360 DNA Polymerase

製品ラインMeltDoctor

製品タイプHrm Reagent Kit

数量1 kit

サンプルタイプDNA (Genomic), cDNA

技術HRM (High Resolution Melting) Analysis, Mutation Scanning

検出法HRM

使用対象（アプリケーション）Real Time PCR (qPCR)

GC-Rich PCR Performance高

標識または色素High Resolution Melting (HRM), SYTO 9

PCR法qPCR

Unit SizeEach

組成および保存条件

This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.

Store at -15° to -25°

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問（FAQ）

HRM解析では何が測定できますか？

HRM解析ではジェノタイピング、Mutationの検索、およびメチル化解析が可能です。

HRM解析を行う際に必要な試薬は何ですか？

HRM解析を行う場合は、装置とソフトウェアのHRM蛍光キャリブレーションを行うための試薬が必要です。

High Resolution Melt (HRM)が使用できる機種は何ですか？

7500F, 7900HT Fast, StepOne/StepOnePlus, QuantStudio 3/5では、HRMソフトウェアを別途ご購入の上、ご利用可能です。以下の機種に関しましては、ソフトウェアにすでに組み込まれているHRMモジュールにて、HRMの機能がご利用可能です。 ViiA7, QuantStudio 6, QuantStudio7, および QuantStudio 12K Flex。HRMモジュールをご使用になる場合は、ライセンスを別途購入する必要があります。

How long should the amplicon be for HRM analysis?

We recommend to use 50-250 bp long PCR amplicons. Typically, shorter amplicons can distinguish the genotypes for a SNP better, especially for Type III and Type IV SNPs. This is simply because a single base variation affects the melting behavior of a 100 bp amplicon stronger than of a 500 bp amplicon, for example. In longer fragments, the risk of covering multiple mutations is also increased.

What can be used as positive control reactions in HRM analysis?

Positive control reactions should contain template DNA with a known sequence. In SNP genotyping experiments, this could be a sample with a known genotype. Positive control(s) for all genotypes should be included where possible to serve as a reference in melting curve comparison and assigning genotypes for test samples. In mutation scanning experiments, a sample with a wild type sequence could serve as a positive control. The controls should preferably have the same DNA concentration as their corresponding test samples. Control DNA should also be eluted and/or diluted in the same buffer as the samples.

View all MeltDoctor™ HRM Reagent Kit FAQs