MeltDoctor™ HRM Calibration Standard (20X)

HRM解析ではジェノタイピング、Mutationの検索、およびメチル化解析が可能です。

HRM解析を行う場合は、装置とソフトウェアのHRM蛍光キャリブレーションを行うための試薬が必要です。

7500F, 7900HT Fast, StepOne/StepOnePlus, QuantStudio 3/5では、HRMソフトウェアを別途ご購入の上、ご利用可能です。以下の機種に関しましては、ソフトウェアにすでに組み込まれているHRMモジュールにて、HRMの機能がご利用可能です。 ViiA7, QuantStudio 6, QuantStudio7, および QuantStudio 12K Flex。HRMモジュールをご使用になる場合は、ライセンスを別途購入する必要があります。

We recommend to use 50-250 bp long PCR amplicons. Typically, shorter amplicons can distinguish the genotypes for a SNP better, especially for Type III and Type IV SNPs. This is simply because a single base variation affects the melting behavior of a 100 bp amplicon stronger than of a 500 bp amplicon, for example. In longer fragments, the risk of covering multiple mutations is also increased.

Positive control reactions should contain template DNA with a known sequence. In SNP genotyping experiments, this could be a sample with a known genotype. Positive control(s) for all genotypes should be included where possible to serve as a reference in melting curve comparison and assigning genotypes for test samples. In mutation scanning experiments, a sample with a wild type sequence could serve as a positive control. The controls should preferably have the same DNA concentration as their corresponding test samples. Control DNA should also be eluted and/or diluted in the same buffer as the samples.