Applied Biosystems™

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

製品番号(カタログ番号): 4426715
Applied Biosystems™

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

製品番号(カタログ番号): 4426715
製品番号(カタログ番号)
4426715
ユニットサイズ
1 kit
価格(JPY)
在庫と納期
製品番号(カタログ番号)ユニットサイズ価格(JPY)在庫と納期
44267151 kit
製品概要
ビデオ
推奨事項
推奨事項
参考資料
FAQ
引用および参考文献
その他の情報
推奨事項
The PrepSEQ™ Spin Sample Preparation Kit – Extra Clean with Proteinase K is recommended for difficult to lyse bacteria, like Listeria monocytogenes, that also has a high lipid content, such as soft cheeses. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

仕様

フォーマット
Spin Column
Isolation Technology
Spin Column, Coarse Filtration, Enzymatic
サンプルタイプ
Swab, Bacteria (Gram +/-), Bacterial Culture, Bacteria (Hard-to-Lyse), Food (High Lipid Content), Water
最終産物タイプ
Bacterial DNA
使用対象 (装置)
Centrifuge
使用対象(アプリケーション)
Real-Time Quantitative PCR (qPCR), PCR
高スループット適合性
Not High-throughput Compatible (Manual)
反応数
100 Reactions
製品ライン
PrepSEQ™
ターゲット
Genomic DNA
出発物質量
750 μL

組成および保存条件

Each kit contains 100 spin columns, 200 microcentrifuge tubes, Lysis Buffer (store at 4°C), and Proteinase K (store at -20°C). 100 samples can be prepared using this kit.

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