Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step Kit

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the詳細を見る
製品番号(カタログ番号)反応数
4428206反応100回分
4428207500 Reactions
44400221000 Reactions
製品番号(カタログ番号) 4428206
価格(JPY)
-
お問い合わせください ›
反応数:
反応100回分
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan® primer⁄probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold® DNA Polymerase, and a buffer mix.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
フォーマットTube
ホットスタート内蔵ホットスタート
反応数反応100回分
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインPath-ID
製品タイプOne-Step RT-PCR Kit
数量100 reactions
逆転写酵素ArrayScript™
サンプルタイプTotal RNA, Viral RNA, mRNA
対応可能対象100反応
検出法Primer-probe
使用対象(アプリケーション)Pathogen Detection, Virus Detection
形状液体
PCR法1-step RT-qPCR, Multiplex qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
• 1.375 mL of 2X Multiplex RT-PCR Buffer
• 280 μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
• 1.75 mL of Nuclease-free water
• Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.