The ViiA™ 7 System TaqMan™ Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems™ ViiA™ 7 Real-Time PCR Systems with the TaqMan™ Low Density Array Block Upgrade.
The ViiA™ 7 System TaqMan™ Low Density Array Instrument Verification RNase P kit contains an empty TaqMan™ Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan™ Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.
The ViiA™ 7 System TaqMan™ Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems™ recommends that the performance of your instrument be verified every six months.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
校正タイプRNase P Calibration, Instrument Verification Plate
使用対象(アプリケーション)Real Time PCR (qPCR)
使用対象 (装置)ViiA™ 7 System
フォーマット384-well Microfluidic Card
製品ラインViiA
数量1 kit
製品タイプArray Card RNaseP Verification Kit
Unit SizeEach
組成および保存条件
8 x Tubes with Standard Dilutions, Empty Array Card, Port 1 NTC, Port 2 Unknown A, Port 3 U, Store at -15°C to -25°C
よくあるご質問(FAQ)
When do I need to run the RNaseP calibration?
The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.
When do I need to run the RNaseP calibration for the QuantStudio 12K Flex Real-Time PCR System?
The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.
Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?
It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system
Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".
Can I import a standard curve for my real-time PCR data into the QuantStudio Real-Time PCR Software?
If you are analyzing results from the ViiA 7 Real-Time PCR System, QuantStudio 6 Flex Real-Time PCR System, or QuantStudio 7 Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Real-Time PCR Software following the instructions below:
1. Open the data file in the QuantStudio Real-Time PCR Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.