Can I set up and store the TaqMan Fast Universal PCR Master Mix at room temperature if the instrument is in use?
For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.
Can I run a reaction made with the standard TaqMan Universal PCR Master Mix in fast thermal cycling mode?
No, we do not recommend this approach. Since the standard TaqMan Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.
Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?
The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.