Path-ID™ Multiplex One-Step RT-PCR Kit
Path-ID™ Multiplex One-Step RT-PCR Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step RT-PCR Kit

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the詳細を見る
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製品番号（カタログ番号）反応数
4442135反応100回分
4442136500 Reactions
44421371000 Reactions
製品番号（カタログ番号） 4442135
価格（JPY）
60,400
Each
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反応数:
反応100回分
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer-probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.
仕様
使用対象 (装置)7500 Fast System, 7500 System
フォーマットTube
ホットスタート内蔵ホットスタート
反応数反応100回分
受動的参照色素ROX（プレミックス）
ポリメラーゼAmpliTaq Gold DNA Polymerase
製品ラインPath-ID
製品タイプMultiplex One-Step RT-PCR Kit
数量100 reactions
逆転写酵素ArrayScript™ UP
サンプルタイプRNA
対応可能対象100反応
検出法Primer-probe
使用対象（アプリケーション）Pathogen Detection, Virus Detection
GC-Rich PCR Performance低
PCR法1-step RT-qPCR, Multiplex qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Store at -20°C. Includes 100 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex Enzyme Mix, and Nuclease-free water.

よくあるご質問（FAQ）

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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