Ion Xpress™ Barcode Adapters 81-96 Kit
Ion Xpress™ Barcode Adapters 81-96 Kit
Ion Torrent™

Ion Xpress™ Barcode Adapters 81-96 Kit

The Ion Xpress™ Barcode Adapters 81-96 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for詳細を見る
製品番号(カタログ番号)数量
44745211 kit
製品番号(カタログ番号) 4474521
価格(JPY)
298,400
Each
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数量:
1 kit

The Ion Xpress™ Barcode Adapters 81-96 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Features of the Ion Xpress™ Barcode Adapters 81-96 Kit include:

  • Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
  • Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
  • Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput

Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design

Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
ライブラリFragment Library
製品タイプBarcode Adapter Kit
数量1 kit
シーケンスタイプGenome & DNA Sequencing
出荷条件Dry Ice
スループット96 Samples per Run
ワークフローステップLibrary Barcoding
製品ラインIon Torrent
原料DNA
Unit SizeEach
組成および保存条件
1 box of 17 tubes, store -20°C

よくあるご質問(FAQ)

Ion Xpress Plus Fragment Library Kit (Cat. No. 4471269) および Ion Plus Fragment Library Kit (Cat. No. 4471252) のユーザーガイドには、 low (50-100 ng) およびhigh (1 µg) DNA inputのプロトコルがありますが、どちらに従えばよいでしょうか?

DNA inputの量は、サンプルからどのくらいのDNA量が抽出できるのか、どのようなアプリケーションに興味があるのかによって決まります。例えば、多様性に富んだライブラリーを作製したい場合(脊椎動物のゲノムライブラリーなど)は、1 μg のDNA inputから始めていただき、超音波で断片化を行い(バイアスを軽減するため)、ライブラリー増幅を行わない(多様性を維持するため)ことをお勧めします。

input量の少ないプロトコル (50-100 ng)は、ampliconライブラリーなど多様性が少ないライブラリーやDNAの収量の少ないサンプルに使用します。100 ng以下のDNA inputの場合、最終的なライブラリー増幅が必要になる可能性があります。また、特定のアプリケーションはlow DNA inputには対応していなく、high DNA inputが必要となる(TargetSeq、ChIP-Seq、Mate-Pairedライブラリーなど)ことにご注意ください。

Do libraries constructed with the Ion Xpress Barcode adapters require balancing to help ensure equal representation on the Ion PGM or Proton instrument sequencing run?

No, the Ion Xpress Barcode adapters are designed with a 3 bp adapter sequence (GAT) at the 3' end of each barcode to help minimize adapter ligation bias. When pooling barcoded libraries, accurate library quantification is extremely important to help ensure even barcode representation on the sequencing run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I am planning to use the Oncomine BRCA Research Assay, Manual Library Preparation (Cat. No. A32840). Which other products would need I to order?

Here are the products you would need to order:

- Ion Xpress Barcode Adapters Kits
- Agencourt AMPure XP (Beckman Coulter Cat. No. A63880 or A63881)
- Ion Library Equalizer Kit (Cat. No. 4482298)