CaptureSelect™ FcXP Pre-packed Column

CaptureSelect FcXP Affinity Matrix is offered here in a pre-packed format with connectors that are compatible with standard chromatography equipment.

CaptureSelect FcXP Affinity Matrix is the successor to CaptureSelect FcXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4). But the matrix features an increased binding capacity (>40 g IgG/L), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix.

Features of this affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived

CaptureSelect FcXP pre-packed columns can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enable a robust and efficient purification process with excellent purity in one step.

Characteristics:

• Matrix: agarose-based, epoxide activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect FcXP affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl₂, 40% (v/v) propylene glycol, pH 7
• Flow characteristics: 150–300 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol