I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?
Our options will depend on the samples you are analyzing.
If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.
For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can the eFluor Organic fluorochromes be used for intracellular staining?
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?
Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?
The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What is the difference between eFluor Organic Dyes and eVolve Qdots?
The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
