Citations & References (6)

Thermo Scientific™

KingFisher™ mL 磁性粒子プロセッサ

Thermo Scientific™ KingFisher mL精製システムを使用して、ラボのワークフロー内のロースループットサンプル調製を自動化します。

製品番号（カタログ番号）	数量
5400050	1台

製品番号（カタログ番号） 5400050

価格（JPY）

1,740,000

非常に高い柔軟性を備えたThermo Scientific™ KingFisher mL精製システムを使用して、ラボのワークフロー内のロースループットサンプル調製を自動化します。最大1 mLの処理容量に適したこの磁性粒子プロセッサによって、5連チューブのストリップ1本ですべての精製ステップが可能であり、1回で15個の処理サンプルに対応できます。ターゲット分子の溶離に50 μLまで容量を低減でき、DNAやRNAなどのサンプルの単離と大容量からの濃縮を同時に行うことができます。

機能：

幅広い引き続く実験アプリケーションのために、高品質な DNA、RNA、タンパク質、および細胞の迅速かつ再現性ある精製を行えます。
フレキシブルなオープンシステムにより、アプリケーションに合わせてあらゆる磁性粒子ベースのキットの使用が可能です
使いやすいThermo Scientific™ BindIt™ソフトウェアにより、機器のコントロールやプロトコルの作成および修正を行えます
さまざまなアプリケーション向けの既存プロトコルを利用可能

推奨用途：

ゲノミクスおよびプロテオミクス、創薬、法医学、バイオマーカー発見、品質管理、獣医学アッセイ

研究用途にのみご使用ください。診断目的には使用できません。

Collection Efficiency of Particles95%

奥行き（ヤードポンド法）11.4 in.

奥行き（メートル法）29 cm

概要KingFisher mL精製システム、100～240 V、50/60 Hz（公称）

ディスプレイSTART (開始)/STOP (停止)/2 つのカーソルキー/LCD

電気的要件100～240 V、50/60 Hz

使用対象（アプリケーション）ゲノミクスおよびプロテオミクス、創薬、法医学、バイオマーカー発見、品質管理、獣医学アッセイ

高さ（ヤードポンド法）12.2 in.

高さ（メートル法）31 cm

磁性ロッド3 x 5 フォーマット

粒子径（メートル法）約>1 μm

チップコンボ特別デザイン、1 x 5 フォーマット

容器タイプ専用チューブストリップ、1 x 5 本

重量（ヤードポンド法）23ポンド

重量（メートル法）10kg

幅（ヤードポンド法）11.4 in.

幅（メートル法）29 cm

容量1回の処理あたり15サンプル

容量（メートル法）処理50～1000 μL

Unit SizeEach

よくあるご質問（FAQ）

Do you have scripts for using the MagMAX kits on the KingFisher mL instrument?

We do not have prewritten scripts for using MagMAX kits on the KingFisher mL instrument. We recommend adapting scripts written for the KingFisher Flex or KingFisher Presto instruments. You will still need to validate the scripts on your KingFisher mL instrument.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I use the MagMAX Express-96 Deep Well Head (Cat. No. 4388435) on the KingFisher 96 instrument?

Yes, the MagMAX Express-96 Deep Well Head can be used on the KingFisher 96 instrument. However, it is not compatible with the KingFisher Flex instrument.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What should I do if the tip comb does not load correctly onto my Thermo Scientific KingFisher system?

If the tip does not load correctly onto a Thermo Scientific KingFisher magnetic head, we recommend that you try manually flexing the tips both lengthwise and widthwise to level the tip comb. A video of flexing the tip comb can be found here: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems/resources.html. The video is under "KingFisher Flex" and titled "How to install the magnet head and check the tip comb." Flexing of the tip comb is demonstrated at timepoint 1:35. If that does not work, try a different box or lot of tips. This applies to all KingFisher instruments.

引用および参考文献 (6)

引用および参考文献

Abstract

Characterization of human cytochrome P450s involved in the bioactivation of tri-ortho-cresyl phosphate (ToCP).

Authors:Reinen J, Nematollahi L, Fidder A, Vermeulen NP, Noort D, Commandeur JN

Journal:

PubMed ID:25706813

'Tri-ortho-cresyl phosphate (ToCP) is a multipurpose organophosphorus compound that is neurotoxic and suspected to be involved in aerotoxic syndrome in humans. It has been reported that not ToCP itself but a metabolite of ToCP, namely, 2-(ortho-cresyl)-4H-1,2,3-benzodioxaphosphoran-2-one (CBDP), may be responsible for this effect as it can irreversibly bind to human ... More

Characterization of the postsynaptic protein neurogranin in paired cerebrospinal fluid and plasma samples from Alzheimer's disease patients and healthy controls.

Authors:Kvartsberg H, Portelius E, Andreasson U, Brinkmalm G, Hellwig K, Lelental N, Kornhuber J, Hansson O, Minthon L, Spitzer P, Maler JM, Zetterberg H, Blennow K, Lewczuk P

Journal:

PubMed ID:26136856

'Synaptic dysfunction and degeneration are central events in Alzheimer''s disease (AD) pathophysiology that are thought to occur early in disease progression. Synaptic pathology may be studied by examining protein biomarkers specific for different synaptic elements. We recently showed that the dendritic protein neurogranin (Ng), including the endogenous Ng peptide 48 ... More

Brain amyloid-beta fragment signatures in pathological ageing and Alzheimer's disease by hybrid immunoprecipitation mass spectrometry.

Authors:Portelius E, Lashley T, Westerlund A, Persson R, Fox NC, Blennow K, Revesz T, Zetterberg H

Journal:

PubMed ID:25591542

'Senile plaques in Alzheimer''s disease (AD) are composed of amyloid-ß (Aß), especially N-truncated forms including Aß4-42. These are thought to be neurotoxic. However, individuals may live for decades with biomarker evidence of cerebral ß-amyloidosis (positive amyloid PET imaging and/or low cerebrospinal fluid levels of the 42 amino acid form of ... More

APP metabolism regulates tau proteostasis in human cerebral cortex neurons.

Authors:Moore S, Evans LD, Andersson T, Portelius E, Smith J, Dias TB, Saurat N, McGlade A, Kirwan P, Blennow K, Hardy J, Zetterberg H, Livesey FJ

Journal:

PubMed ID:25921538

'Accumulation of Aß peptide fragments of the APP protein and neurofibrillary tangles of the microtubule-associated protein tau are the cellular hallmarks of Alzheimer''s disease (AD). To investigate the relationship between APP metabolism and tau protein levels and phosphorylation, we studied human-stem-cell-derived forebrain neurons with genetic forms of AD, all of ... More

Highly potent soluble amyloid-ß seeds in human Alzheimer brain but not cerebrospinal fluid.

Authors:Fritschi SK, Langer F, Kaeser SA, Maia LF, Portelius E, Pinotsi D, Kaminski CF, Winkler DT, Maetzler W, Keyvani K, Spitzer P, Wiltfang J, Kaminski Schierle GS, Zetterberg H, Staufenbiel M, Jucker M

Journal:

PubMed ID:25212850

The soluble fraction of brain samples from patients with Alzheimer's disease contains highly biologically active amyloid-ß seeds. In this study, we sought to assess the potency of soluble amyloid-ß seeds derived from the brain and cerebrospinal fluid. Soluble Alzheimer's disease brain extracts were serially diluted and then injected into the ... More

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