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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts.
Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details)
Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
仕様
細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
細胞タイプPrimary Rat Astrocytes
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプMEM (Minimum Essential Medium)
数量10 L
品質保持期間36 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Powder
Serum LevelStandard Serum Supplementation
無菌性Sterile-filtered
添加剤ありLow Glucose, Glutamine, Phenol Red
添加剤なしNo HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture