Citations & References (10)

Thermo Scientific™

Hoechst 33342 solution (20 mM), 5 mL

Thermo Scientific Pierce Hoechst 33342蛍光染色剤は、細胞イメージング技術におけるDNAと核の固定およびライブセル蛍光染色用のHoechst色素の高品質溶液です。Hoechst 33342蛍光染色剤の特長：•DNA（真核細胞の核）に特異的なHoechst色素—青色蛍光染色剤•詳細を見る

製品番号（カタログ番号）	数量
62249	5 mL

製品番号（カタログ番号） 62249

価格（JPY）

28,500

Thermo Scientific Pierce Hoechst 33342蛍光染色剤は、細胞イメージング技術におけるDNAと核の固定およびライブセル蛍光染色用のHoechst色素の高品質溶液です。

Hoechst 33342蛍光染色剤の特長：

•DNA（真核細胞の核）に特異的なHoechst色素—青色蛍光染色剤
• 便利—使いやすいHoechst染料溶液（20 mM）として提供されています
• 細胞イメージング—細胞透過性色素は、固定細胞や生細胞の染色に有効です
• 蛍光顕微鏡や高含有量スクリーニング（HCS）のための蛍光抗体による特定のターゲットの検出と並行して使用するのに最適な対比染色—

Hoechst 33342（2'-[4-エトキシフェニル]-5-[4-メチル-1-ピペラジニル]-2,5'-bi-1H-ベンズイミダゾール三塩酸塩三水和物）は、紫外線で励起され、460～490 nmで青色の蛍光を発する細胞透過性DNA染色剤です。Hoechst 33342は、DNA のアデニン-チミン（A-T）領域に優先的に結合します。この染色剤は、DNAの副溝に結合し、色素・塩基対比に依存した明瞭な蛍光発光スペクトルを示します。

Hoechst蛍光色素の特性
蛍光イメージングにおける対比染色として、Hoechst染料は、フルオレセインおよびローダミン色素で標識された抗体や他のプローブだけでなく、Thermo Scientific DyLight Fluorsと互換性があります。安定した20 mM水溶液は、基本的にすぐに使用できます。

Hoechst 33342は、生細胞や固定細胞や組織の核を特異的に染色するために使用されます。この染色剤は通常、アポトーシス核の凝縮クロマチンを識別し、複製細胞を同定し、そのDNA含有量に基づいて細胞を分類するために、5-ブロモ-2'-デオキシウリジン（BrdU）ラベリングと組み合わせて使用されます。Hoechst 33342およびヨウ化プロピジウムは、アポトーシスと細胞周期分布の段階の同時フローサイトメトリーと蛍光イメージング分析によく使用されています。

研究用途にのみご使用ください。診断目的には使用できません。

濃度20 mM

検出法蛍光

染色剤タイプ細胞透過性

発光497 nm

励起波長域361⁄497

使用対象（アプリケーション）Fluorescent labeling

使用対象 (装置)蛍光顕微鏡、ハイコンテント機器

形状Liquid

製品ラインPierce

数量5 mL

出荷条件Room Temperature

標識タイプFluorescent Dye

製品タイプ核酸染色

Sub Cellular Localization核

Unit SizeEach

組成および保存条件

保存:受領後、4°Cで保存し、遮光してください。製品は室温で出荷されます。

よくあるご質問（FAQ）

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?

Platelet cells don't have DNA, while bacteria do. Therefore, a cell-permeant, DNA-selective dye would preferentially stain the bacteria with limited staining of the platelets. We recommend using Hoechst 33342 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (10)

引用および参考文献

Abstract

Generation, transcriptome profiling, and functional validation of cone-rich human retinal organoids.

Authors:Kim S, Lowe A, Dharmat R, Lee S, Owen LA, Wang J, Shakoor A, Li Y, Morgan DJ, Hejazi AA, Cvekl A, DeAngelis MM, Zhou ZJ, Chen R, Liu W

Journal:Proc Natl Acad Sci U S A

PubMed ID:31072937

'Rod and cone photoreceptors are light-sensing cells in the human retina. Rods are dominant in the peripheral retina, whereas cones are enriched in the macula, which is responsible for central vision and visual acuity. Macular degenerations affect vision the most and are currently incurable. Here we report the generation, transcriptome ... More

Yin Yang 1 Orchestrates a Metabolic Program Required for Both Neural Crest Development and Melanoma Formation.

Authors:Varum S, Baggiolini A, Zurkirchen L, Atak ZK, Cantù C, Marzorati E, Bossart R, Wouters J, Häusel J, Tuncer E, Zingg D, Veen D, John N, Balz M, Levesque MP, Basler K, Aerts S, Zamboni N, Dummer R, Sommer L

Journal:Cell Stem Cell

PubMed ID:30951662

'Increasing evidence suggests that cancer cells highjack developmental programs for disease initiation and progression. Melanoma arises from melanocytes that originate during development from neural crest stem cells (NCSCs). Here, we identified the transcription factor Yin Yang 1 (Yy1) as an NCSCs regulator. Conditional deletion of Yy1 in NCSCs resulted in ... More

Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides.

Authors:Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K

Journal:Elife

PubMed ID:29809136

We introduce a new class of semisynthetic fluorescent biosensors for the quantification of free nicotinamide adenine dinucleotide (NAD

Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.

Authors:Buhr ED, Vemaraju S, Diaz N, Lang RA, Van Gelder RN

Journal:Curr Biol

PubMed ID:31607531

Nearly all mammalian tissues have functional, autonomous circadian clocks, which free-run with non-24 h periods and must be synchronized (entrained) to the 24 h day. This entrainment mechanism is thought to be hierarchical, with photic input to the retina entraining the master circadian clock in the suprachiasmatic nuclei (SCN) and the SCN ... More

Authors:Li S, Lavagnino Z, Lemacon D, Kong L, Ustione A, Ng X, Zhang Y, Wang Y, Zheng B, Piwnica-Worms H, Vindigni A, Piston DW, You Z

Journal:Mol Cell

PubMed ID:31053472

Abnormal processing of stressed replication forks by nucleases can cause fork collapse, genomic instability, and cell death. Despite its importance, it is poorly understood how the cell properly controls nucleases to prevent detrimental fork processing. Here, we report a signaling pathway that controls the activity of exonuclease Exo1 to prevent ... More

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